Cross-correlation-based transverse flow measurements using optical resolution photoacoustic microscopy with a digital micromirror device
Cross-correlation-based transverse flow measurements using optical resolution photoacoustic microscopy with a digital micromirror device
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DOI：
10.1117/1.jbo.18.9.096004
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发表时间：
2013-09-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Liang, Jinyang;Zhou, Yong;Wang, Lihong V.
提出了一种基于互相关的方法，利用数字微镜增强的光声显微术定量测量横向流速。DMD用于交替地将两个空间分离的激光束传送到目标。通过互相关的慢时间PA从两个光束测量的轮廓之间，横向流的速度和方向同时来自的幅度和符号的时间偏移，分别。在0.50至6.84毫米/秒的范围内的横向流动准确地测量使用10微米直径的微球的水悬浮液，和均方根测量精度被量化为0.22毫米/秒。在0.55至6.49 mm/s的速度范围内的流量测量是独立的颗粒大小的流量，这是实验证明使用三种不同尺寸的微球（直径：3，6，和10 μ m）。测得的流速沿垂直于水流的深度方向沿着遵循预期的抛物线分布。两个最大和最小可测量的速度进行了调查，为两个光束之间的不同距离和不同的总时间为一个测量。该技术在0.3 mm深度处散射鸡胸肉的精度为0.35 mm/s，使其有希望用于测量生物组织中的流量。(C)2013年，美国光电仪器工程师学会（SPIE）
A cross-correlation-based method is proposed to quantitatively measure transverse flow velocity using optical resolution photoacoustic (PA) microscopy enhanced with a digital micromirror device (DMD). The DMD is used to alternately deliver two spatially separated laser beams to the target. Through cross-correlation between the slow-time PA profiles measured from the two beams, the speed and direction of transverse flow are simultaneously derived from the magnitude and sign of the time shift, respectively. Transverse flows in the range of 0.50 to 6.84 mm/s are accurately measured using an aqueous suspension of 10-mu m-diameter microspheres, and the root-mean-squared measurement accuracy is quantified to be 0.22 mm/s. The flow measurements are independent of the particle size for flows in the velocity range of 0.55 to 6.49 mm/s, which was demonstrated experimentally using three different sizes of microspheres (diameters: 3, 6, and 10 mu m). The measured flow velocity follows an expected parabolic distribution along the depth direction perpendicular to the flow. Both maximum and minimum measurable velocities are investigated for varied distances between the two beams and varied total time for one measurement. This technique shows an accuracy of 0.35 mm/s at 0.3-mm depth in scattering chicken breast, making it promising for measuring flow in biological tissue. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)