The Comparison of Fecal Microbiota in Left-Side and Right-Side Human Colorectal Cancer
The Comparison of Fecal Microbiota in Left-Side and Right-Side Human Colorectal Cancer
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DOI：
10.1159/000516922
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发表时间：
2021-07-01
影响因子：
1.6
通讯作者：
Tani, Masaji
中科院分区：
文献类型：
--
作者：
Miyake, Toru;Mori, Haruki;Tani, Masaji
简介：微生物组在癌症的发展和进展中起着至关重要的作用。临床状态，包括预后，基因突变和对化疗的敏感性，取决于结直肠癌（CRC）的位置;然而，肠道微生物群与CRC位置之间的关联尚未完全了解。这项研究的目的是根据肿瘤的位置来评估CRC患者肠道微生物群的差异。方法：研究纳入了2018年8月至2019年11月期间接受CRC手术的五十六例患者。排除了3例术前1个月内接受过新辅助治疗或抗生素治疗的患者。使用16 s rRNA扩增子序列的V3-V4区域评估术前粪便中微生物群的宏基因组。结果：左侧CRC的Bray-Curtis距离的β多样性显著高于右侧CRC。根据线性判别分析效应量方法，梭杆菌在左侧CRC中占主导地位。布劳特氏菌属、赤藓菌属、霍尔德氏菌属、粪杆菌属、亚长粒菌属和多雷亚菌属构成了右侧CRC的优势肠道植物群。途径分析显示，L-赖氨酸发酵和cob（II）yrinate a，c-二酰胺生物合成I在左侧CRC中占主导地位。讨论：本研究表明，左侧CRC的粪便微生物群在组成和功能上与右侧CRC不同。这些结果将有助于阐明根据肿瘤位置的生物学差异，并开发人类CRC的治疗方法。
Introduction: Microbiomes play a vital role in the development and progression of cancer. The clinical status, including prognosis, genetic mutations, and sensitivity to chemotherapy, differs depending on the location of colorectal cancer (CRC); however, the association between gut microbiota and the location of CRC is not entirely understood. This study was conducted to evaluate the differences in the gut microbiota in patients with CRC according to the location of the tumor. Methods: Fifty-six patients who underwent surgery for CRC between August 2018 and November 2019 were included in the study. Three patients who had received neoadjuvant therapy or antibiotic treatment within 1 month before surgery were excluded. The metagenomes of microbiota in preoperative feces were assessed using the V3-V4 region of 16s rRNA amplicon sequences. Results: The beta diversity of the Bray-Curtis distance was significantly higher in left-sided than in right-sided CRC. Fusobacterium predominated in left-sided CRC according to the linear discriminant analysis effect size method. Blautia, Eryspelotrichales, Holdemanella, Faecalibacterium, Subdoligranulum, and Dorea constituted the dominant intestinal flora in right-sided CRC. Pathway analysis revealed that L-lysine fermentation and cob(II)yrinate a,c-diamide biosynthesis I were predominant in left-sided CRC. Discussion: This study demonstrated that fecal microbiota in left-sided CRC constitutionally and functionally differ from those in right-side CRC. These results will help to elucidate the biological differences according to tumor location and develop treatments for human CRC.