Use of picosecond Kerr-gated Raman spectroscopy to suppress signals from both surface and deep layers in bladder and prostate tissue.
Use of picosecond Kerr-gated Raman spectroscopy to suppress signals from both surface and deep layers in bladder and prostate tissue.
复制标题
使用皮秒克尔门控拉曼光谱抑制膀胱和前列腺组织表面和深层的信号。
DOI：
--
复制
发表时间：
2005
影响因子：
3.5
通讯作者：
N. Stone
中科院分区：
文献类型：
--
作者：
Maria Consuelo Hart Prieto;P. Matousek;M. Towrie;A. Parker;Mark Wright;A. Ritchie;N. Stone
拉曼光谱是一种光学技术，能够询问生物组织，让我们了解与疾病发展相关的分子结构变化。克尔选通拉曼光谱技术使用皮秒脉冲激光以及对收集的拉曼散射光进行快速时间选通。本研究的前列腺样本取自经尿道前列腺电切术(TURP)的芯片，膀胱样本取自TURBT和TURP的活检术。通过膀胱和前列腺组织获得的光谱，在激光脉冲后的不同时间延迟，清楚地显示光谱随着深度剖析的发生而变化，最终分别显示来自尿酸细胞和尿素细胞的信号。我们第一次展示了，使用这项新技术，我们能够通过前列腺和膀胱获得不同深度的光谱。这对拉曼光谱作为一种诊断工具的未来具有重大意义。在拉曼光谱和克尔门控的帮助下，有可能从良性腺体中前列腺癌的一个小病灶中提取光谱差异，并通过评估切除后肿瘤的基础来对膀胱癌进行分期。
Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman scattered light. Prostate samples for this study were obtained by taking a chip at the transurethral resection of the prostate (TURP), and bladder samples from a biopsy taken at transurethral resection of bladder tumor (TURBT) and TURP. Spectra obtained through the bladder and prostate gland tissue, at different time delays after the laser pulse, clearly show change in the spectra as depth profiling occurs, eventually showing signals from the uric acid cell and urea cell, respectively. We show for the first time, using this novel technique, that we are able to obtain spectra from different depths through both the prostate gland and the bladder. This has major implications in the future of Raman spectroscopy as a tool for diagnosis. With the help of Raman spectroscopy and Kerr gating, it may be possible to pick up the spectral differences from a small focus of adenocarcinoma of the prostate gland in an otherwise benign gland, and also stage the bladder cancers by assessing the base of the tumor post resection.