Low abundance of respiratory nitrate reductase is essential for Escherichia coli in resistance to aminoglycoside and cephalosporin
Low abundance of respiratory nitrate reductase is essential for Escherichia coli in resistance to aminoglycoside and cephalosporin
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DOI：
10.1016/j.jprot.2013.05.019
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发表时间：
2013-07-11
影响因子：
3.3
通讯作者：
Peng, Xuan-xian
中科院分区：
文献类型：
--
作者：
Ma, Yan;Guo, Chang;Peng, Xuan-xian
在本研究中，我们的特点是低丰度的NarG和NarH，呼吸道硝酸还原酶（Nar）的两个组成部分，链霉素（SM）-，庆大霉素（GEN）-，头孢他啶（CAZ）-，四环素（泰特）-和萘啶酸（NA）耐药大肠杆菌菌株使用天然/SDS-PAGE蛋白质组学。我们使用蛋白质印迹和native/SDS-PAGE对narG和narH缺失突变体验证了这一发现。然而，进一步的功能证据表明，narG和narH的缺失导致两种类型的生长行为，高于和低于对照，在这些抗虫E。大肠杆菌菌株。具体而言，SM-，GEN-和CAZ-耐药细菌生长更快，而NA-和TET-耐药大肠杆菌。大肠杆菌菌株生长较慢。我们的数据表明，低丰度的呼吸Nar是必要的E。大肠埃希菌对氨基糖苷类和头孢菌素类抗生素耐药。同时，研究结果表明，不同的耐药菌存在不同的耐药机制。NA抗性和TET抗性大肠杆菌逆转生长的原因是：大肠杆菌菌株有待调查。我们的研究结果有助于提出新的战略，
In the present study, we have characterized low abundance of NarG and NarH, two components of respiratory nitrate reductase (Nar), in streptomycin (SM)-, gentamicine (GEN)-, ceftazidime (CAZ)-, tetracycline (TET)- and nalidixic acid (NA)-resistant Escherichia coli strains using native/SDS-PAGE based proteomics. We validate the finding using Western blotting and native/SDS-PAGE upon narG and narH deletion mutants. However, further functional evidence indicates that loss of narG and narH results in two types of growth behaviors, higher and lower than control, in these antibiotic-resistant E. coli strains. Specifically, SM-, GEN- and CAZ-resistant bacteria grow faster, whereas NA- and TET-resistant E. coli strains grow slower. Our data indicate that low abundance of respiratory Nar is essential for E. coli in resistance to aminoglycoside and cephalosporin antibiotics. Meanwhile, the results show that differential mechanisms exist in different antibiotic-resistant bacteria. The reason why the reversal growths are detected in NA- and TET-resistant E. coli strains waits investigation. Our findings serve to propose novel strategies fo