Comparison of human colonic mucoprotein antigen from normal and neoplastic mucosa.
Comparison of human colonic mucoprotein antigen from normal and neoplastic mucosa.
复制标题
正常粘膜和肿瘤粘膜的人结肠粘蛋白抗原的比较。
作者：
D. Gold;F. Miller
摘要结肠粘蛋白抗原（CMA）是一种高分子量的组织特异性粘蛋白，从9例正常粘膜和12例结肠腺癌中获得。通过苯酚：水萃取，然后乙醇沉淀和分子筛色谱法实现纯化。对单个正常CMA制剂的化学分析表明，定性和定量成分相似。分子的肽部分的特征在于高含量的苏氨酸、丝氨酸、脯氨酸、谷氨酸和甘氨酸。寡糖链由N -羟乙酰神经氨酸、岩藻糖、半乳糖、葡萄糖胺和半乳糖胺组成。正常和肿瘤CMA的比较揭示了以下差异。虽然氨基酸组成相似，但肿瘤CMA 9的苏氨酸比正常组织的CMA 9少40%，天冬氨酸多40%。两个观察结果均具有统计学显著性，
Abstract Colonic mucoprotein antigen (CMA), a high-molecularweight, tissue-specific mucin, was obtained from 9 normal mucosal samples and 12 colonic adenocarcinomas. Purification was achieved by means of phenol:water extraction followed by ethanol precipitation and molecular sieve chromatography. Chemical analyses of the individual normal CMA preparations indicated similar qualitative and quantitative compositions. The peptide portion of the molecule was characterized by a high content of threonine, serine, proline, glutamic acid, and glycine. The oligosaccharide chains were composed of N -glycolyineuraminic acid, fucose, galactose, glucosamine, and galactosamine. A comparison of the normal and tumor CMA revealed the following differences. Although the amino acid compositions were similar, tumor CMA9s had 40% less threonine and 40% more aspartic acid than did CMA9s from normal tissue. Both observations were statistically significant at 2 p p