Redirecting Human Conventional and Regulatory T Cells Using Chimeric Antigen Receptors.
Redirecting Human Conventional and Regulatory T Cells Using Chimeric Antigen Receptors.
复制标题
使用嵌合抗原受体重定向人类常规和调节性 T 细胞。
DOI：
10.1007/978-1-0716-3593-3_15
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Ferreira,LeonardoMR
中科院分区：
文献类型：
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作者：
Zimmerman,CapersM;Robino,RobA;Cochrane,RussellW;Dominguez,MatthewD;Ferreira,LeonardoMR
适应性免疫系统表现出精致的特异性和记忆，几乎参与人体的每一个过程。将适应性免疫细胞（特别是T细胞）重新定向到所需的靶点，有可能为各种疾病创造强大的基于细胞的疗法。虽然常规效应T细胞（Teff）将靶向待消除的细胞，如癌细胞，但免疫抑制调节性T细胞（Treg）将针对待保护的组织，如移植器官。嵌合抗原受体（汽车）是设计分子，其包含细胞外识别结构域和细胞内信号传导结构域，其直接驱动靶标结合下游的完全T细胞活化。在这里，我们描述了产生和评估人CAR CD4+辅助T细胞、CD8+细胞毒性T细胞和CD4+FOXP3+调节性T细胞的程序。
The adaptive immune system exhibits exquisite specificity and memory and is involved in virtually every process in the human body. Redirecting adaptive immune cells, in particular T cells, to desired targets has the potential to lead to the creation of powerful cell-based therapies for a wide range of maladies. While conventional effector T cells (Teff) would be targeted towards cells to be eliminated, such as cancer cells, immunosuppressive regulatory T cells (Treg) would be directed towards tissues to be protected, such as transplanted organs. Chimeric antigen receptors (CARs) are designer molecules comprising an extracellular recognition domain and an intracellular signaling domain that drives full T cell activation directly downstream of target binding. Here, we describe procedures to generate and evaluate human CAR CD4+helper T cells, CD8+cytotoxic T cells, and CD4+FOXP3+regulatory T cells.