Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes
Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes
复制标题
DOI：
10.1093/jac/dks114
复制
发表时间：
2012-07-01
影响因子：
5.2
通讯作者：
Carattoli, Alessandra
中科院分区：
文献类型：
--
作者：
Villa, Laura;Poirel, Laurent;Carattoli, Alessandra
为了表征从来自摩洛哥的序列型（ST）15的肺炎克雷伯氏菌分离株回收的质粒pNDM-MAR，其携带编码金属-β-内酰胺酶NDM-1的基因，超广谱内酰胺酶（ESBL）CTX-M-15和qnrB 1喹诺酮耐药决定簇。基因组测序仪FLX程序对从大肠杆菌J53转移接合子获得的总质粒DNA构建的文库进行分析。pNDM-MAR质粒全长267242 bp，编码177个预测蛋白。它窝藏新的复制子和转移位点，定义了一个新的质粒类型的IncH质粒家族。bla（NDM-1）基因的侧翼是与在其他bla（NDM-1）阳性质粒中观察到的遗传元件不同的遗传元件，包括groES和groEL伴侣蛋白基因。该质粒携带ESBL基因bla（CTX-M-15）和喹诺酮类耐药基因qnrB 1。此外，它还含有编码抗亚碲酸盐、汞、氯霉素和氨基糖苷类药物的基因。有趣的是，pNDM-MAR不携带任何16 S rRNA甲基化酶基因，与其他bla（NDM-1）阳性质粒相反，这项研究强调了参与bla（NDM-1）基因传播的遗传媒介的多样性。质粒pNDM-MAR与所有已知的携带bla（NDM-1）的质粒显著不同。pNDM-MAR序列的比较分析鉴定了一种新型的IncH质粒。
To characterize plasmid pNDM-MAR recovered from a Klebsiella pneumoniae isolate of sequence type (ST) 15 from Morocco, carrying the genes encoding the metallo--lactamase NDM-1, the extended-spectrum -lactamase (ESBL) CTX-M-15 and the qnrB1 quinolone resistance determinant.The plasmid DNA sequence was obtained by using the 454-Genome Sequencer FLX procedure on a library constructed from total plasmid DNA obtained from an Escherichia coli J53 transconjugant. Contig assembly and predicted gaps were confirmed and filled by PCR-based gap closure.Plasmid pNDM-MAR was 267242 bp long and encoded 177 predicted proteins. It harboured novel replicons and transfer loci, defining a novel plasmid type within the IncH plasmid family. The bla(NDM-1) gene was flanked by genetic elements that are distinct from those observed in other bla(NDM-1)-positive plasmids, including the groES and groEL chaperonin genes. This plasmid harboured the ESBL gene bla(CTX-M-15) together with the quinolone resistance gene qnrB1. In addition, it harboured genes encoding resistance to tellurite, mercury, chloramphenicol and aminoglycosides. Interestingly, pNDM-MAR did not carry any 16S rRNA methylase gene, in contrast to other bla(NDM-1)-positive plasmids.This study underlines the diversity of genetic vehicles involved in the spread of the bla(NDM-1) gene. Plasmid pNDM-MAR differed significantly from all known bla(NDM-1)-bearing plasmids. Comparative analysis of the pNDM-MAR sequence identified a novel type of IncH plasmid.