Gap site-specific rapid formation of fluorescent silver nanoclusters for label-free DNA nucleobase recognition.
Gap site-specific rapid formation of fluorescent silver nanoclusters for label-free DNA nucleobase recognition.
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DOI：
10.1016/j.aca.2012.02.044
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发表时间：
2012-04
影响因子：
6.2
通讯作者：
Qinghua Cui;Kun Ma;Yong Shao;Shu-juan Xu;Fei Wu;Guiying Liu;N. Teramae;H. Bao
中科院分区：
文献类型：
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作者：
Qinghua Cui;Kun Ma;Yong Shao;Shu-juan Xu;Fei Wu;Guiying Liu;N. Teramae;H. Bao
以dna为模板的银纳米团簇作为一种新型荧光基团，由于其易于通过模板dna的序列和长度进行发射调节而备受关注。然而，以位点特异性的方式精确生产Ag nc仍然是实现高选择性和无标记DNA识别的挑战。在这里，我们利用DNA双链中的间隙位点作为合成Ag nc的新支架。与常用的DNA模板相比，发现DNA双链中的间隙位点有利于快速形成荧光Ag nc，而不会牺牲其明亮的发光和优异的稳定性。我们发现，当胞嘧啶朝向DNA双链的间隙位点时，荧光Ag NCs是高度选择性形成的，并且它们被原位用作DNA突变分析的信号接通方式的读数。这种在缺口位点的碱基选择性生长的荧光Ag NCs将在单核苷酸多态性（SNP）的实际检测和基于dna的功能传感器的构建中找到有前途的应用，具有无标记和成本效益的优点。
Silver nanoclusters (Ag NCs) templated with DNAs have attracted much attention as novel fluorophores because of their convenient emission tunability by the sequence and length of the template DNAs. However, the precise production of Ag NCs in a site-specific manner still remains a challenge to attain highly selective and label-free DNA recognition. Here we exploited the availability of a gap site in DNA duplexes as a new scaffold for the synthesis of Ag NCs. Compared to the commonly used DNA templates for the creation of Ag NCs, the gap site in DNA duplexes was found to facilitate the rapid formation of the fluorescent Ag NCs without sacrifice of their bright emission and excellent stability. We found that fluorescent Ag NCs were highly selectively formed when cytosine faced toward the gap site in DNA duplexes, and they were in situ utilized as readout by signal-on manner for the DNA mutation assays. This base-selective growth of the fluorescent Ag NCs at the gap site would find promising applications in practical detection of single nucleotide polymorphism (SNP) and construction of DNA-based functional sensors with label-free and cost-effective merits.