Distribution of the perivascular nerve Ca2+ receptor in rat arteries
Distribution of the perivascular nerve Ca2+ receptor in rat arteries
复制标题
DOI：
10.1038/sj.bjp.0702195
复制
发表时间：
1998-12-01
影响因子：
7.3
通讯作者：
Bukoski, RD
中科院分区：
文献类型：
--
作者：
Wang, YL;Bukoski, RD
1我们最近发现肠系膜分支动脉的血管周围感觉神经表达一种细胞外钙受体(CAR)，报道的数据表明这种CAR介导了生理水平的钙离子引起的松弛。我们现在已经测试了血管周围感觉神经连锁扩张器系统是局限于肠系膜的局部现象，还是存在于其他循环中。2研究了来自肠系膜、肾、冠状动脉和脑循环的血管。用抗CAR和抗神经细胞黏附分子(NCAM)抗体进行免疫细胞化学分析。3尽管所有动脉的血管周围神经都有CAR蛋白染色，但存在区域差异。用形态计量学方法估计CAR阳性神经密度的顺序如下：肠系膜支动脉(GT)；基底动脉=肾叶间动脉(GT)；肾主干动脉(GT)；冠状动脉左前降支。4血管来自肠系膜、肾、冠脉和脑循环，当预先收缩5-羟色胺(5-羟色胺)时，表现出神经依赖性的松弛。钙离子诱导的肠系膜动脉、肾动脉和脑动脉的松弛程度与EFS诱导的松弛程度呈正相关。相反，冠状动脉收缩在1.5~3 mmoL·L~(-1)之间，略有松弛至5 mmo1 L~(-1)。5因此，在肠系膜、肾脏和脑循环中存在不同程度的功能性血管周围感觉神经联系的扩张器系统，但在冠状循环中仅有非常有限的程度。
1 We recently showed that perivascular sensory nerves of mesenteric branch arteries express a receptor for extracellular Ca2+ (CaR), and reported data indicating that this CaR mediates relaxation induced by physiologic levels of Ca2+. We have now tested whether the perivascular sensory nerve CaR-linked dilator system is a local phenomenon restricted to the mesentery, or is present in other circulations.2 Vessels from the mesenteric, renal, coronary, and cerebral circulations were studied. Immunocytochemical analysis was performed using anti-CaR and anti-neural cell adhesion molecule (NCAM) antibodies. Wire myography was used to assess contracation and relaxation.3 Although perivascular nerves of all arteries stained for CaR protein, there were regional differences. A morphometric method used to estimate CaR positive nerve density revealed the following rank order: mesenteric branch artery > basilar artery = renal interlobar artery > main renal trunk artery > left anterior descending coronary artery.4 Vessels from the mesentery, renal, coronary, and cerebral circulations showed nerve-dependent relaxation in response to electrical held stimulation (EFS) when precontracted with serotonin in the presence of guanethidine. The degree of Ca2+-induced relaxation of mesenteric, renal, and cerebral arteries positively correlated with the magnitude of EFS-induced relaxation. In contrast, coronary arteries contracted at Ca2+ levels between 1.5 and 3 mmol L-1,and relaxed to a small degree to 5 mmol L-1 Ca2+.5 Thus, a functional perivascular sensory nerve CaR-linked dilator system is present to varying degrees in the mesenteric, renal, and cerebral circulations, but only to a very limited extent in the coronary circulation.