Co-expression of opsins in the eye photoreceptors of the terrestrial slug Limax valentianus.
Co-expression of opsins in the eye photoreceptors of the terrestrial slug Limax valentianus.
复制标题
陆生蛞蝓 Limax valentianus 眼睛感光器中视蛋白的共表达。
DOI：
10.1002/cne.24732
复制
发表时间：
2019
期刊：
影响因子：
2.5
通讯作者：
and Matsuo Y
中科院分区：
文献类型：
--
作者：
*Matsuo R;Koyanagi M;Nagata A;and Matsuo Y
视蛋白与Gq型G蛋白偶联被认为与软体动物的视觉有关。然而，最近的转录组学研究显示，在软体动物的眼睛中存在属于不同视蛋白亚家族的视蛋白mRNA。在本研究中，我们发现至少有三种不同的视蛋白，Gq偶联的视紫红质，视紫红质5a和异视紫红蛋白，在陆源鼻涕虫（limmax valentianus）眼睛的横纹状光感受器细胞中共表达。这些视蛋白都位于横纹肌的微绒毛上。双荧光原位杂交也证实了视紫红质和opsin5A mRNA的共表达。横纹肌光感受器细胞中多种视蛋白的共同表达可能解释了先前报道的limax flavus眼睛视网膜电图在光适应状态和暗适应状态之间的作用谱变化，这在本研究中也得到了再现。valentianus。
Visual opsins coupled with Gq‐type G protein have been considered to be responsible for the vision in mollusks. Recent transcriptomic studies, however, revealed the presence of opsin mRNA belonging to different groups of opsin subfamilies in the eyes of mollusks. In the present study, we found that at least three different opsins, Gq‐coupled rhodopsin, opsin5A, and xenopsin, are co‐expressed in the rhabdomeric photoreceptor cell in the eyes of the terrestrial slugLimax valentianus. These opsins were all localized to the microvilli of the rhabdomere. Co‐expression of rhodopsin and opsin5A mRNA was also demonstrated by dual fluorescence in situ hybridization. Co‐expression of multiple opsins in the rhabdomeric photoreceptors cells may explain the previously reported shift in the action spectra of the electroretinogram of eyes ofLimax flavusbetween the light‐ and dark‐adapted states, which was also reproduced in the present study inL. valentianus.