Molecular cloning and function analysis of ClCRY1a and ClCRY1b, two genes in Chrysanthemum lavandulifolium that play vital roles in promoting floral transition.
Molecular cloning and function analysis of ClCRY1a and ClCRY1b, two genes in Chrysanthemum lavandulifolium that play vital roles in promoting floral transition.
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DOI：
10.1016/j.gene.2017.02.020
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发表时间：
2017-06
期刊：
影响因子：
3.5
通讯作者：
Liwen Yang;Jianxin Fu;Shuai Qi;Yan Hong;He Huang;S. Dai
中科院分区：
文献类型：
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作者：
Liwen Yang;Jianxin Fu;Shuai Qi;Yan Hong;He Huang;S. Dai
Cryptochrome (CRY), a vital photoreceptor which mediates light signals, controls photomorphogenesis in higher plants. However, the function of CRY in mediating light to regulate growth and development of ornamental plants is still unclear. In this study, we identified twoCRY1homologous genes,ClCRY1aandClCRY1b, fromChrysanthemum lavandulifolium, a diploid wild chrysanthemum species. Expression analysis demonstrated that these twoClCRY1genes showed the highest expression levels in seedlings leaves that were transferred to short day (SD) conditions for eight days.ClCRY1awas expressed at a higher level in the dark phase of SD, whileClCRY1bwas expressed more highly in SD than that in long day (LD) conditions. Overexpression of eitherClCRY1aorClCRY1bin wild-type (WT) Arabidopsis resulted in early flowering under both LD and SD. The expression levels ofGIGANTEA(GI) andFLOWERING LOCUS T(FT) were significantly up-regulated inClCRY1aoverexpressors under both LD and SD. Moreover, the transcript levels ofGI,CONSTANS(CO) andFTwere markedly increased inClCRY1boverexpressors under LD; nevertheless, only the expression levels ofCOandFTwere up-regulated under SD. Taken together, the above results indicated that these twoClCRY1genes could regulate flowering time via different pathways inC.lavandulifoliumunder LD and SD conditions. Our results provided evidence for the role ofClCRY1sin controlling photomorphogenesis and laid a foundation for further study on the molecular mechanism of ClCRYs mediating light signals to control floral transition.
