Membrane Shape Dynamics-Based Analysis of the Physical Properties of Giant Unilamellar Vesicles Prepared by Inverted Emulsion and Hydration Techniques
Membrane Shape Dynamics-Based Analysis of the Physical Properties of Giant Unilamellar Vesicles Prepared by Inverted Emulsion and Hydration Techniques
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DOI：
10.1021/acs.langmuir.0c02698
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发表时间：
2021-02-23
期刊：
影响因子：
3.9
通讯作者：
Noda, Naohiro
中科院分区：
文献类型：
--
作者：
Morita, Masamune;Noda, Naohiro
巨单层囊泡（GUV）是细胞膜的基本模型，其允许调节和控制膜形状动力学，其在活细胞膜的功能中起重要作用。然而，为了正确使用这些人工细胞模型系统，我们需要了解它们的物理特性。GUV生成技术是人工细胞模型系统综合的关键技术。然而，目前尚不清楚不同技术生产的GUV是否具有相同的物理特性。在这里，我们已经研究了通过反相乳液和水合技术制备的GUV的物理性质，通过检查由外部刺激与非离子表面活性剂引起的膜形状变形。我们揭示了膜的自发曲率的差异，膜的内部和外部小叶之间的优选差分区域，以及使用两种不同技术制备的GUV之间的膜孔的边缘张力。
The giant unilamellar vesicle (GUV) is a basic model of the cell membrane that allows for the modulation and control of membrane shape dynamics, which play essential roles in the functions of living cell membranes. However, to properly use these artificial cell-like model systems, we need to understand their physical properties. GUV generation techniques are key technologies in the synthesis of artificial cell-like model systems. However, it is unclear whether GUVs produced by different techniques have the same physical properties. Here, we have investigated the physical properties of GUVs prepared by inverted emulsion and hydration techniques by examining the membrane shape deformation induced by external stimulation with a nonionic surfactant. We reveal differences in the spontaneous curvature of the membrane, the preferred differential area between the inner and outer leaflets of the membrane, and the edge tension of membrane pores between the GUVs prepared using the two distinct techniques.