Immunohistochemical localization of group II phospholipase A2 in the tumours and mucosa of the colon and rectum
Immunohistochemical localization of group II phospholipase A2 in the tumours and mucosa of the colon and rectum
复制标题
DOI：
10.1053/ejso.2001.1134
复制
发表时间：
2001-09-01
期刊：
影响因子：
--
通讯作者：
Golouh, R
中科院分区：
文献类型：
--
作者：
Edhemovic, I;Snoj, M;Golouh, R
背景：Ⅱ型磷脂酶A（2）（PLA（2））是一种在恶性转化和恶性细胞侵袭过程中起重要作用的酶。本研究的目的是研究II组PLA（2）在结直肠癌、癌旁粘膜和粘膜中的表达。方法：对57例大肠癌（盲肠癌10例，升结肠癌10例，横结肠癌10例，乙状结肠癌9例，直肠癌18例）切除标本进行化学成分分析。用人单克隆II组PLA（2）抗体（“Upstate Biotechnology”，Lake Placid，NY 12946，USA）对石蜡块的组织学载玻片进行染色。抗体类别：IgGIK。免疫原：HPC纯化的人精子磷脂酶A（2）- 14 kDa。酶），使用TechMate 500染色仪的标准DAKO过氧化物酶标记的链霉亲和素-印迹法。根据阳性细胞的广泛性和强度半定量地评估II组PLA（2）表达。对于统计学评价，使用Kruskal-Wallis单因素秩和方差分析和Mann-Whitney秩和检验。Ⅱ组PLA（2）在癌周粘膜中表达最高（中位数4.00），在远离肿瘤的粘膜中低得多（中位数0.70），肿瘤本身几乎无活性（中位数0.00），所有差异均具有统计学显著性（所有成对多重比较程序- Dunn方法：P
Background: Group II phospholipase A(2) (PLA(2)) is an enzyme important in malignant transformation and in the invasion process of malignant cells. The aim of the present study was to investigate the expression of group II PLA(2) in the cancers of the colorectum, peritumoural mucosa and in the mucosa. distant from the tumour.Methods: Resection specimens from 57 patients with colorectal carcinoma (caecum 10, ascending 10, transverse 10, sigmoid colon nine, and rectum 18) were analysed immunohistochemically. Histological slides from paraffin blocks were stained by the human monoclonal group II PLA(2) antibody ('Upstate Biotechnology', Lake Placid, NY 12946, USA. Antibody Class: IgGIk. Immunogen: HPC purifed human sperm phospholipase A(2) - 14 kDa. enzyme) using the standard DAKO peroxidase-labelled streptavidin-blotin method by TechMate 500 stainer. Group II PLA(2) expression was evaluated semi-quantitatively according to the extensivity and intensivity of the positive cells. For statistical evaluation the Kruskal-Wallis one way analysis of variance on ranks and the Mann-Whitney rank sum tests were used.Results: The highest expression of group II PLA(2) was found in the peritumoural mucosa (median 4.00), much lower in the mucosa distal from the tumour (median 0.70) and almost no activity in the tumour itself (median 0.00), all differences were statistically significant (all pairwise multiple comparison procedures - Dunn's Method: P