Epidemiology of precore mutants of hepatitis B in the United Kingdom
Epidemiology of precore mutants of hepatitis B in the United Kingdom
复制标题
英国乙型肝炎前核心突变体的流行病学
DOI：
--
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发表时间：
2000
影响因子：
12.7
通讯作者：
E. Boxall
中科院分区：
文献类型：
--
作者：
A. Ballard;E. Boxall
采用点突变法对310例慢性乙型肝炎B携带者（HBeAg阳性82例，HBeAg阴性228例）的28位密码子和1位密码子前C区突变情况进行了研究。228例HBeAg阴性携带者中有14例（6%）血清HBV DNA水平升高。其中9个由前核心变异解释，3个由核心启动子变异解释，2个不由公认的前核心变化解释。巢式PCR检测HBeAg阴性者血清HBVDNA阳性率为36%（82/228），其中前C变异阳性率为63%（52/82）。82例HBeAg阳性携带者中有4例（4%）有前C变异体，均为突变型/野生型混合人群，证据表明这些携带者发生血清转化。23%（52/228）的HBeAg阴性携带者同时存在血清HBV DNA和密码子1或28前C区突变。观察到一名血清HBV DNA水平相对较低（104-106基因组拷贝/ml）且仅有核心启动子突变的HBeAg阴性携带者发生性传播事件。尽管在产前亚组中变异携带率很高，但未观察到围产期传播。对45例携带者的直接测序结果验证了点突变试验的正确性，同时也表明28号密码子突变仅见于密码子15处CCT基因型的携带者。对于高加索人群，观察到密码子28突变的发生率（13/25或52%）高于预期。肝活检数据表明，前C区突变的存在或不存在与肝病的严重程度之间没有联系。医学病毒学杂志62：463-470，2000.© 2000 Wiley利斯公司
A point mutation assay was used to study the codon 28 and codon 1 precore mutant status of 310 chronic hepatitis B carriers (82 HBeAg positive and 228 HBeAg negative). Fourteen of 228 (6%) of HBeAg negative carriers had high levels of serum HBV DNA. Nine of these were explained by precore variants, three by core promoter variants, and two were not explained by recognised precore changes. Nested PCR detected serum HBV DNA in 36% (82/228) of HBeAg negative carriers and 63% (52/82) of these had precore variants. Four of 82 (4%) of the HBeAg positive carriers had precore variants, all as mixed mutant/wild type populations and evidence indicated that these carriers were seroconverting. Overall 23% (52/228) of HBeAg negative carriers had both serum HBV DNA and codon 1 or 28 precore mutations. A sexual transmission event from an HBeAg negative carrier with a relatively low serum HBV DNA level (104–106 genome copies/ml) and only core promoter mutations was observed. Despite high rates of variant carriage in the antenatal sub‐group perinatal transmission was not observed. The results of direct sequencing on 45 carriers validated the point mutation assay and also showed that codon 28 mutations were only seen in carriers with the genotype CCT at codon 15. For the Caucasian population a higher prevalence of codon 28 mutations (13/25 or 52%) than expected was seen. Liver biopsy data indicated that there was no link between the presence or absence of precore mutants and the severity of liver disease. J. Med. Virol. 62:463–470, 2000. © 2000 Wiley‐Liss, Inc.
影响因子：
15.9
作者：
Baumert, TF;Rogers, SA;Liang, TJ
通讯作者：
Liang, TJ
血清中乙型肝炎病毒 DNA 的酶扩增与黑猩猩感染性测试的比较。
DOI：
10.1093/infdis/160.1.37
发表时间：
1989
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Ulrich,PP;Bhat,RA;Seto,B;Mack,D;Sninsky,J;Vyas,GN
通讯作者：
Vyas,GN