Clinical application of immunocytochemical detection of ALK rearrangement on cytology slides for detection or screening of lung adenocarcinoma
Clinical application of immunocytochemical detection of ALK rearrangement on cytology slides for detection or screening of lung adenocarcinoma
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免疫细胞化学检测细胞学载玻片上ALK重排在肺腺癌检测或筛查中的临床应用
DOI：
10.1016/j.lungcan.2013.03.006
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发表时间：
2013
期刊：
影响因子：
5.3
通讯作者：
Kurose A
中科院分区：
文献类型：
--
作者：
Tanaka H;Tone K;Hayashi A;Morimoto T;Taima K;Tanaka Y;Nakagawa H;Takanashi S;Okumura K;Kurose A
间变性淋巴瘤激酶（ALK）重排的免疫组化筛查已被认为是必要的，并定期进行肺腺癌的治疗选择。然而，经支气管肺活检（TBLB）难以接近肿瘤，它往往无法获得肿瘤组织，而肿瘤细胞包含在同时获得的细胞学标本时，支气管镜检查。因此，我们对同一病例的TBLB标本采用免疫组化（IHC）和刷片细胞学切片免疫细胞化学（ICC）检测ALK蛋白的表达，并比较IHC和ICC结果的一致率。经细胞学诊断和脱色后，在常规巴氏染色的切片上进行ICC。结果：在广崎大学医院和广崎国立医院提取了18例腺癌患者。免疫组化和免疫组化结果的一致率很高：免疫组化和免疫组化结果的敏感性为85.7%（6/7），特异性为100%（11/11），阳性预测值为100%（6/6），阴性预测值为91.6%（11/12）。在常规巴氏细胞学切片上使用ICC检测ALK重排被认为有利于肺癌治疗。
Immunohistochemical screening of Anaplastic lymphoma kinase (ALK) rearrangement has been regarded essential and routinely carried out to select treatment for lung adenocarcinoma. However, difficulty to approach a tumor by transbronchial lung biopsy (TBLB), it often fails to obtain tumor tissues whereas tumor cells are contained in cytology specimens simultaneously obtained when the bronchoscopy is done. Therefore we evaluated the expression of ALK protein by using immunohistochemistry (IHC) on TBLB specimens and immunocytochemistry (ICC) on brushing smear cytology slides in the same cases, and compared the concordance rate of IHC and ICC results. ICC was carried out on routine Papanicolau-stained slides after cytology diagnosis and decolorization. RESULTS: Eighteen patients with adenocarcinoma were extracted in the Hirosaki University Hospital and the Hirosaki National Hospital. IHC and ICC results showed a very high concordance rate: sensitivity of ICC in comparison with IHC was 85.7% (6/7), specificity was 100% (11/11), positive predictive value was 100% (6/6), and negative predictive value was 91.6% (11/12). Detection of ALK rearrangement using ICC on routine Papanicolau cytology slides is considered to be advantageous for lung cancer treatments.