Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast
Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast
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DOI：
10.1093/emboj/21.4.602
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发表时间：
2002-02-15
期刊：
影响因子：
11.4
通讯作者：
Gerst, JE
中科院分区：
文献类型：
--
作者：
Gurunathan, S;David, D;Gerst, JE
酵母产生两类分泌囊泡（SV），它们在密度和货物蛋白含量上都不同。在迟效分泌突变体（如snc 1（ala 43）和sec 6 -4），低密度（LDSV）和高密度（HDSV）类囊泡积累在限制性温度。在这里，我们发现，在编码动力蛋白相关蛋白（VPS 1）或网格蛋白重链（CHC 1）的基因中断废除HDSV的生产，产生LDSV含有所有分泌的货物。有趣的是，破坏PEP 12基因（其编码介导所有高尔基体到前液泡区室（PVC）运输的t-SNARE）也会消除HDSV的产生。相比之下，选择性地赋予液泡水解酶分选到PVC或蛋白质运输到液泡的基因（即分别为VPS 34和VAM 3）中的缺失没有影响。因此，酵母分泌途径的一个分支涉及中间分选室，并在SV生物发生中对网格蛋白和发动蛋白相关蛋白有特定的需求。
Yeast produce two classes of secretory vesicles (SVs) that differ in both density and cargo protein content. In late-acting secretory mutants (e.g. snc1(ala43) and sec6-4), both low- (LDSV) and high-density (HDSV) classes of vesicles accumulate at restrictive temperatures. Here, we have found that disruptions in the genes encoding a dynamin-related protein (VPS1) or clathrin heavy chain (CHC1) abolish HDSV production, yielding LDSVs that contain all secreted cargos. Interestingly, disruption of the PEP12 gene, which encodes the t-SNARE that mediates all Golgi to prevacuolar compartment (PVC) transport, also abolishes HDSV production. In contrast, deletions in genes that selectively confer vacuolar hydrolase sorting to the PVC or protein transport to the vacuole (i.e. VPS34 and VAM3, respectively) have no effect. Thus, one branch of the secretory pathway in yeast involves an intermediate sorting compartment and has a specific requirement for clathrin and a dynamin-related protein in SV biogenesis.