Combined approaches to site-specific modification of RNA
Combined approaches to site-specific modification of RNA
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DOI：
10.1021/cb7002225
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发表时间：
2008-01-01
影响因子：
4
通讯作者：
Lamichhane, Tek N.
中科院分区：
文献类型：
--
作者：
Chow, Christine S.;Mahto, Santosh K.;Lamichhane, Tek N.
RNA中的天然和非天然修饰都是生物学家和化学家感兴趣的。在自然界中已经鉴定了四种标准RNA核苷的100多种不同类似物。非天然修饰对于RNA的结构和机制研究是有用的。这篇评论强调了化学，酶，和组合（半合成）的方法来产生位点特异性修饰的RNA。为了研究RNA化学组成、结构和功能之间的关系，这些方法对所有大小的RNA的位点特异性修饰的可用性是重要的。
Both natural and unnatural modifications in RNA are of interest to biologists and chemists. More than 100 different analogues of the four standard RNA nucleosides have been identified in nature. Unnatural modifications are useful for structure and mechanistic studies of RNA. This Review highlights chemical, enzymatic, and combined (semisynthesis) approaches to generate site specifically modified RNAs. The availability of these methods for site-specific modifications of RNAs of all sizes is important in order to study the relationships between RNA chemical composition, structure, and function.