Recombinant protein, AlnA, combined with transgenic alfalfa remediates polychlorinated biphenyl-contaminated soils: efficiency and rhizosphere microbial community response
Recombinant protein, AlnA, combined with transgenic alfalfa remediates polychlorinated biphenyl-contaminated soils: efficiency and rhizosphere microbial community response
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重组蛋白 AlnA 与转基因苜蓿结合修复多氯联苯污染的土壤：效率和根际微生物群落反应
DOI：
10.1007/s10529-016-2169-1
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Yan Wang
中科院分区：
文献类型：
--
作者：
Hejun Ren;Yu Su;Junxing Zhang;Hongyu Pan;Baoyu Chen;Yan Wang
目的研究生物乳化蛋白AlNA与表达bphC的紫花苜蓿复合修复多氯联苯污染土壤的效果。结果通过盆栽试验，AlNA与转基因紫花苜蓿的结合促进了多氯联苯土壤修复。转基因紫花苜蓿/Alna处理对三氯联苯(PCB16/PCB32和PCB31/PCB28)和四联苯(PCB49)的去除效率分别是单独使用转基因紫花苜蓿的3.6、1.1和2倍。基因拷贝数分析表明，转基因紫花苜蓿/Alna处理中类假单胞菌的多氯联苯降解基因bphc是对照土壤的1.5倍。细菌群落Illumina测序显示假单胞菌、节杆菌和鞘氨醇单胞菌与多氯联苯的降解率呈正相关。结论多氯联苯的去除与细菌群落多样性无关，但与其特异性降解物和bphC基因的表达呈正相关。
ObjectiveTo investigate the remediation efficiency of polychlorinated biphenyl (PCB)-contaminated soils by the combination of a bioemulsifying protein, AlnA, and alfalfa expressingbphC.ResultThe combination of AlnA and transgenic alfalfa promoted PCB soil remediation through the pot experiments. The removal rates of tri-PCBs (PCB 16/PCB 32 and PCB 31/PCB 28) and tetra-PCB (PCB 49) in transgenic alfalfa/AlnA treatment were 3.6-, 1.1-, and 2-fold higher than in transgenic alfalfa treatment alone. Analysis of gene copy number revealed that the PCB-degrading gene,bphC,ofPseudomonas-like bacterial populations in transgenic alfalfa/AlnA treatment increased 1.5-fold compared with that of unplanted soils. Bacterial community Illumina sequencing showedPseudomonas,Arthrobacter, andSphingomonaspositively correlated with the removal rates of PCBs.ConclusionsPCB removal was unrelated to bacterial community diversity but positively correlated with their specific degraders andbphCgene expression.
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DOI：
10.5860/choice.31-3799
发表时间：
2013-01
期刊：
--
影响因子：
--
作者：
S. Pal
通讯作者：
S. Pal
影响因子：
11.8
作者：
Yalin Wang;Jinxing Hu;Wei Lin;Ning Wang;Cheng Li;P. Luo;M. Z. Hashmi;Wenbo Wang;X. Su;Cheng Chen;Yindong Liu;Ronglang Huang;Chaofeng Shen
通讯作者：
Yalin Wang;Jinxing Hu;Wei Lin;Ning Wang;Cheng Li;P. Luo;M. Z. Hashmi;Wenbo Wang;X. Su;Cheng Chen;Yindong Liu;Ronglang Huang;Chaofeng Shen
影响因子：
3.8
作者：
Novakova, M.;Mackova, M.;Macek, T.
通讯作者：
Macek, T.
DOI：
--
发表时间：
2006-02
期刊：
Lawrence Berkeley National Laboratory
影响因子：
--
作者：
T. DeSantis;P. Hugenholtz;N. Larsen;M. Rojas;Eoin L. Brodie;K. Keller;T. Huber;D. Dalevi;
通讯作者：
T. DeSantis;P. Hugenholtz;N. Larsen;M. Rojas;Eoin L. Brodie;K. Keller;T. Huber;D. Dalevi;
DOI：
10.1016/j.scitotenv.2009.11.031
发表时间：
2010-02
期刊：
The Science of the total environment
影响因子：
--
作者：
Li Xu;Ying Teng;Zhen-gao Li;J. Norton;Yong-ming Luo
通讯作者：
Li Xu;Ying Teng;Zhen-gao Li;J. Norton;Yong-ming Luo