Benzamide prevention of ultraviolet radiation-induced transformation as measured by anchorage-independent growth and the absence of correlation with thymidine dimer formation and DNA repair.
Benzamide prevention of ultraviolet radiation-induced transformation as measured by anchorage-independent growth and the absence of correlation with thymidine dimer formation and DNA repair.
复制标题
苯甲酰胺可预防紫外线辐射诱导的转化（通过不依赖锚定的生长来衡量），并且与胸苷二聚体形成和 DNA 修复不存在相关性。
DOI：
10.1002/tcm.1770090305
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Kun,E
中科院分区：
文献类型：
--
作者：
Milo,GE;D'Ambrosio,S;Kun,E
Synchronized human fibroblasts were exposed in early S phase to increasing doses of ultraviolet (UV) irradiation in the presence and absence of an antitrans‐forming drug, benzamide. Cellular survival, initial thymidine dimer formation and its repair, and cellular phenotypic transformation were simultaneously monitored in the presence and absence of 1 mM externally added benzamide that reaches 8 to 15 μM intracellular levels. Cellular transformation as measured by an expression of anchorage‐independent growth was inhibited by nontoxic doses of benzamide. Antitransforming action of benzamide is confined to low intracellular drug concentrations, which in the case of benzamide is in the 4–9 μM range. Because of the lack of effect of benzamide on the formation of UV‐induced thymidine dimers and the specific repair of these dimers, these results suggest that the processes of thymidine dimer formation and its repair are not involved in the mode of action of benzamide that influences the expression of a transformed phenotype with low malignant vigor.
