A REVIEW OF WATER DIFFUSION MEASUREMENT BY NMR IN HUMAN RED BLOOD-CELLS
A REVIEW OF WATER DIFFUSION MEASUREMENT BY NMR IN HUMAN RED BLOOD-CELLS
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DOI：
10.1152/ajpcell.1989.256.5.c1097
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发表时间：
1989-05-01
影响因子：
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通讯作者：
GOLDSTEIN, JH
中科院分区：
文献类型：
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作者：
HERBST, MD;GOLDSTEIN, JH
本文对正常人红细胞水转运的测量进行了综述，试图用两种不同的技术来协调在不同研究条件下得到的不一致的结果：核磁共振(核磁共振)和放射性示踪扩散(THO)。核磁共振实验结果之间的大部分差异似乎是由红细胞自然聚集成圆环造成的。其余的差异归因于使用了不适当的数学近似的两位交换方程，血液储存时间的差异，以及未能调整非水质子的核磁共振计算。红细胞压积、频率-磁场强度或核磁共振脉冲技术的差异对核磁共振报告之间的差异没有明显作用。剔除这些混杂因素后，核磁共振或放射性示踪剂流入或整体扩散获得的扩散结果在相对较窄的数值范围内符合。这些技术将在室温(20-25摄氏度)下新鲜的正常人红细胞内水的平均寿命置于9.8到14毫秒的极端之间，以前未校正的范围是9.8-21.7ms。这一新的水交换次数范围对应于扩散渗透率在3.3~4.7×10(-3)cm/S之间的范围。
This review of water transport measurement in normal human erythrocytes attempts to harmonize discordant results obtained under diverse study conditions with two different techniques: nuclear magnetic resonance (NMR) and radioactive tracer (THO) diffusion. Natural aggregation of red cells into rouleaux appeared to cause most of the variation among results from NMR experiments. The remainder of the discrepancy was attributed to the use of inappropriate mathematical approximations of the two-site exchange equations, differences in blood storage time, and failure to adjust NMR calculations for the nonwater protons. Differences in hematocrit, frequency-magnetic field strength, or NMR pulse technique played no apparent role in the disparity among NMR reports. When these confounding factors were removed, diffusion results obtained by NMR or by influx or bulk diffusion of radioactive tracer agreed within a relatively narrow range of values. These techniques place the mean lifetime of water inside fresh normal human erythrocytes at room temperature (20-25 degrees C) between the extremes of 9.8 and 14 ms, where the uncorrected range was previously 9.8-21.7 ms. This new range of water exchange times corresponds to a range of diffusional permeability between 3.3 and 4.7 x 10(-3) cm/s.