Rac function and regulation during Drosophila development
Rac function and regulation during Drosophila development
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DOI：
10.1038/416438a
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发表时间：
2002-03-28
期刊：
影响因子：
64.8
通讯作者：
Dickson, BJ
中科院分区：
文献类型：
--
作者：
Hakeda-Suzuki, S;Ng, J;Dickson, BJ
Rac GTPases 调节肌动蛋白细胞骨架以控制细胞形状的变化(1,2)。迄今为止，对发育过程中 Rac 功能的分析在很大程度上依赖于显性突变亚型的使用。在这里，我们利用功能缺失突变来证明果蝇的三个 Rac 基因 Rac1、Rac2 和 Mtl 在控制上皮形态发生、成肌细胞融合以及轴突生长和引导方面具有重叠的功能。正如根据使用显性突变亚型的研究所建议的那样，它们不是建立平面细胞极性所必需的(3,4)。鸟嘌呤核苷酸交换因子 Trio 对于轴突生长和引导的 Rac 功能至关重要，但对于上皮形态发生或成肌细胞融合则不然。因此，不同的 Rac 激活剂在不同的发育过程中发挥作用。对 Rac 激活的特异性细胞反应可能更多地由上游激活剂决定，而不是所涉及的特定 Rac 蛋白。
Rac GTPases regulate the actin cytoskeleton to control changes in cell shape(1,2). To date, the analysis of Rac function during development has relied heavily on the use of dominant mutant isoforms. Here, we use loss-of-function mutations to show that the three Drosophila Rac genes, Rac1, Rac2 and Mtl, have overlapping functions in the control of epithelial morphogenesis, myoblast fusion, and axon growth and guidance. They are not required for the establishment of planar cell polarity, as had been suggested on the basis of studies using dominant mutant isoforms(3,4). The guanine nucleotide exchange factor, Trio, is essential for Rac function in axon growth and guidance, but not for epithelial morphogenesis or myoblast fusion. Different Rac activators thus act in different developmental processes. The specific cellular response to Rac activation may be determined more by the upstream activator than the specific Rac protein involved.