Elicitor induction of mRNA activity. Rapid effects of elicitor on phenylalanine ammonia-lyase and chalcone synthase mRNA activities in bean cells.
Elicitor induction of mRNA activity. Rapid effects of elicitor on phenylalanine ammonia-lyase and chalcone synthase mRNA activities in bean cells.
复制标题
mRNA 活性的诱导子诱导。
DOI：
10.1111/j.1432-1033.1983.tb07127.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Christopher J. Lamb
中科院分区：
文献类型：
--
作者：
Michael A. Lawton;Richard A. Dixon;Klaus Hahlbrock;Christopher J. Lamb
以矮生菜豆（Phaseolus vulgaris L.）细胞为材料，研究了诱导子诱导下苯丙烷生物合成的两个特征酶--苯丙氨酸解氨酶和查尔酮合成酶mRNA活性的变化。已研究了免疫沉淀的[35 S]蛋氨酸标记的酶亚基在mRNA依赖性兔网织红细胞裂解物翻译系统中体外合成。诱导剂热释放从细胞壁的炭疽病的病原体菜豆Colletotrichum lindemuthianum，引起显着的快速增加的多核糖体活性的mRNA编码的两种酶伴随着酶活性的快速增加的阶段，在菜豆蛋白积累的发病过程中的植物防御素防御反应。激发子处理后30分钟，可以观察到编码这两种酶的多聚体mRNA活性增加。mRNA活性的诱导模式在时间和激发子浓度方面大致相似，尽管在给予最大诱导的激发子浓度中观察到酶之间的小但明显的差异。在诱导动力学和对诱导剂浓度的依赖性方面，多核糖体mRNA活性的诱导和通过诱导剂处理的体内酶合成的诱导之间存在密切的相关性。这些数据表明，激发子刺激体内苯丙氨酸解氨酶和查耳酮合酶的合成主要是由于编码这些酶的mRNA的多核糖体活性增加。从各种来源的激发子制剂中观察到类似的多核糖体mRNA活性诱导模式。编码苯丙氨酸解氨酶和查耳酮合酶的多核糖体mRNA活性的显著增加作为总细胞mRNA活性的比例而增加，表明激发子不通过刺激从细胞mRNA的总库中选择性募集来增加这些多核糖体mRNA活性。
Changes in the activity levels of mRNAs encoding phenylalanine ammonia-lyase and chalcone synthase, two characteristic enzymes of phenylpropanoid biosynthesis, in elicitor-treated cells of dwarf French bean (Phaseolus vulgaris L.) have been investigated by immunoprecipitation of [35S]methionine-labelled enzyme subunits synthesised in vitro in an mRNA-dependent rabbit reticulocyte lysate translation system. Elicitor heat-released from cell walls of Colletotrichum lindemuthianum, the causal agent of anthracnose disease of bean, causes marked rapid increases in the polysomal activities of the mRNAs encoding the two enzymes concomitant with the phase of rapid increase in enzyme activity at the onset of phaseollin accumulation during the phytoalexin defence response. Increased polysomal mRNA activities encoding the two enzymes can be observed 30 min after elicitor treatment. The patterns of induction of the mRNA activities are broadly similar with respect to time and elicitor concentration although small but distinct differences between the enzymes were observed in the elicitor concentration giving maximum induction. There is a close correlation between the induction of polysomal mRNA activity and the induction of enzyme synthesis in vivo by elicitor treatment with respect to both the kinetics of induction and the dependence on elicitor concentration. The data indicate that elicitor stimulation of phenylalanine ammonia-lyase and chalcone synthase synthesis in vivo is largely a result of increased polysomal activity of the mRNAs encoding these enzymes. Similar patterns of induction of polysomal mRNA activity are observed with elicitor preparations from a variety of sources. The marked increases in polysomal mRNA activities encoding phenylalanine ammonia-lyase and chalcone synthase are increases as a proportion of total cellular mRNA activity, indicating that elicitor does not increase these polysomal mRNA activities by stimulation of selective recruitment from the total pool of cellular mRNA.