Chemical genoprotection: reducing biological damage to as low as reasonably achievable levels
Chemical genoprotection: reducing biological damage to as low as reasonably achievable levels
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DOI：
10.1259/dmfr/95408354
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发表时间：
2011-07-01
影响因子：
3.3
通讯作者：
Canteras, M.
中科院分区：
文献类型：
--
作者：
Alcaraz, M.;Armero, D.;Canteras, M.
目的：本研究旨在评估人类饮食中具有抗突变保护能力的抗氧化物质，以对抗因接触X射线所诱导的基因毒性损伤，试图将生物损伤合理地降低到尽可能低的水平。
方法：使用淋巴细胞胞质分裂阻滞微核（MN）细胞组学试验对10种化合物进行评估。在暴露于2戈瑞X射线照射前5分钟，将所研究的化合物以25微摩尔的浓度加入人血中。
结果：根据X射线暴露所产生的微核频率，所评估的抗氧化物质的保护能力从高到低依次为：迷迭香酸＞5 - 鼠尾草酸 = δ - 生育酚 = L - 抗坏血酸 = 芹菜素 = 氨磷汀（P＜0.001）＞绿茶提取物 = 地奥司明 = 芦丁 = 二甲基亚砜（P＜0.05）＞照射对照组。在所给予的辐射剂量下，基因毒性损伤的降低达到58%，这代表着X射线诱导的染色体损伤显著降低（P＜0.001）。这种保护程度大于氨磷汀所获得的保护程度，氨磷汀是一种用于放射治疗的放射防护化合物，其特点是毒性较高。
结论：几种抗氧化物质是人类饮食中的常见成分且无毒性，可提供对电离辐射所诱导的生物危害的保护。在进行放射检查前应考虑给患者使用这些保护物质，即使是在小辐射剂量的情况下，也不论预期的生物损伤如何。《口腔颌面放射学》（2011年）40卷，310 - 314页。doi:10.1259/dmfr/95408354
Objectives: The aim of this study was to evaluate the antioxidant substances present in the human diet with an antimutagenic protective capacity against genotoxic damage induced by exposure to X-rays in an attempt to reduce biological damage to as low a level as reasonably possible.Methods: Ten compounds were assessed using the lymphocyte cytokinesis-block micronucleus (MN) cytome test. The compounds studied were added to human blood at 25 mu M 5 min before exposure to irradiation by 2 Gy of X-rays.Results: The protective capacity of the antioxidant substances assessed was from highest to lowest according to the frequency of the MN generated by X-ray exposure: rosmarinic acid 5 carnosic acid = delta-tocopherol = L-acid ascorbic = apigenin = amifostine (P < 0.001) > green tea extract = diosmine = rutin = dimetylsulfoxide (P < 0.05) > irradiated control. The reduction in genotoxic damage with the radiation doses administered reached 58%, which represents a significant reduction in X-ray-induced chromosomal damage (P < 0.001). This degree of protection is greater than that obtained with amifostine, a radioprotective compound used in radiotherapy and which is characterised by its high toxicity.Conclusion: Several antioxidant substances, common components of the human diet and lacking toxicity, offer protection from the biological harm induced by ionizing radiation. Administering these protective substances to patients before radiological exploration should be considered, even in the case of small radiation doses and regardless of the biological damage expected. Dentomaxillofacial Radiology (2011) 40, 310-314. doi: 10.1259/dmfr/95408354