Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design
Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design
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DOI：
10.1007/978-1-4939-7295-1_2
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Arnold, Frances H.
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文献类型：
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作者：
Cahn, Jackson K. B.;Brinkmann-Chen, Sabine;Arnold, Frances H.
烟酰胺腺嘌呤二核苷酸 (NAD) 或烟酰胺腺嘌呤二核苷酸磷酸 (NADP) 作为氧化还原载体的酶的特异性可能会给代谢工程和合成生物学应用带来重大障碍，而在这些应用中，切换特异性可能是有益的。我们开发了一种易于使用的计算工具（CSR-SALAD），用于设计突变体库，以简化逆转酶辅因子特异性的过程。在这里，我们描述了该工具的最佳使用方式，并介绍了其在实验室环境中的应用方法。
The specificity of enzymes for nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) as redox carriers can pose a significant hurdle for metabolic engineering and synthetic biology applications, where switching the specificity might be beneficial. We have developed an easy-to-use computational tool (CSR-SALAD) for the design of mutant libraries to simplify the process of reversing the cofactor specificity of an enzyme. Here, we describe the optimal use of this tool and present methods for its application in a laboratory setting.