Genome-wide analysis of spatial and temporal gene expression in rice panicle development
Genome-wide analysis of spatial and temporal gene expression in rice panicle development
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DOI：
10.1111/j.1365-313x.2006.02703.x
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发表时间：
2006-05-01
期刊：
影响因子：
7.2
通讯作者：
Kyozuka, J
中科院分区：
文献类型：
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作者：
Furutani, I;Sukegawa, S;Kyozuka, J
水稻花序（穗）的基本结构是由分支形成的模式决定的，这是在穗发育的早期阶段建立的。在这项研究中，我们对水稻穗发育的早期阶段从相变到花器官分化进行了全球转录组分析。为了生成分生组织特异性基因表达谱，人工收集了极年轻的茎尖分生组织（sam）和随后形成的分生组织，并用于cDNA微阵列分析。我们从22000个基因中鉴定出357个在穗发育早期阶段表达差异的基因，并根据它们的时间表达模式将357个基因分为7组。最显著的特征是，在相变后，少量转录因子广泛富集的基因在SAM中立即上调。原位杂交分析表明，所分析的每个基因都表现出独特而有趣的mRNA定位。值得注意的是，其中一个转录因子被证明是一个密切的下游组分，其中LAX是穗分支的主要调节剂。这些结果表明，我们的策略-仔细收集分生组织，全球转录组分析和随后的原位杂交分析-不仅有助于获得基因表达的全基因组视图，而且有助于揭示控制水稻穗发育的遗传网络。
The basic structure of a rice inflorescence (the panicle) is determined by the pattern of branch formation, which is established at the early stages of panicle development. In this study we conducted global transcriptome profiling of the early stages of rice panicle development from phase transition to floral organ differentiation. To generate a meristem-specific gene-expression profile, shoot apical meristems (SAMs) and subsequently formed, very young panicles were collected manually and used for cDNA microarray analysis. We identified 357 out of 22 000 genes that are expressed differentially in the early stages of panicle development, and the 357 genes were classified into seven groups based on their temporal expression patterns. The most noticeable feature is that a fairly small number of genes, which are extensively enriched in transcription factors, are upregulated in the SAM immediately after phase transition. In situ hybridization analysis showed that each gene analysed exhibits a unique and interesting localization of mRNA. Remarkably, one of the transcription factors was proven to be a close downstream component of the pathway in which LAX, a major regulator of panicle branching, acts. These results suggest that our strategy - careful collection of meristems, global transcriptome analysis and subsequent in situ hybridization analysis - is useful not only to obtain a genome-wide view of gene expression, but also to reveal genetic networks controlling rice panicle development.