Cloning and expression of cold-inducible RNA binding protein in domestic yak (Bos grunniens)
Cloning and expression of cold-inducible RNA binding protein in domestic yak (Bos grunniens)
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家牦牛冷诱导RNA结合蛋白的克隆与表达
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
J. He
中科院分区：
文献类型：
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作者：
K. Yang;Q. Zhang;Z. Wen;Y. Pan;S. Yu;J. He
冷诱导RNA结合蛋白（CIRP）在低温等应激过程中过表达，并参与调节低温适应。本研究采用相对定量真实的时间聚合酶链反应（RT-PCR）、Western blot和免疫组织化学方法，从mRNA和蛋白水平检测CIRPin.adult牦牛心、肝、脾、肺、肾、脑、卵巢、睾丸和皮肤中的表达。从家牦牛脑中克隆了CIRP开放阅读框架编码区。RT-PCR和Western blot结果显示，CIRP在心脏、肾脏、脑、睾丸和皮肤中表达量较高，在肺中表达量较低。免疫组织化学染色显示CIRP表达于神经元细胞、精原细胞、初级精母细胞和表皮细胞的细胞核及残留组织的胞浆中。这些结果为进一步了解CIRP蛋白在牦牛高原适应性长期进化中的重要作用提供了新的资料。
Cold-inducible RNA binding protein (CIRP) is over-expressed during cold and.many other stresses, and could regulate the adaptation to hypothermia. In the.present investigation, the objective was to determine the expression of CIRP in.adult yak heart, liver, spleen, lung, kidney, brain, ovary, testis and skin by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot and.immunohistochemistry from mRNA and protein levels. The CIRP open reading.frame encoding was cloned from the domestic yak brain. Results of RT-PCR and.Western blot showed the high expression level of CIRP in the heart, kidney, brain,.testis and skin, and the lower expression level of CIRP in the lung. Immunohistochemical staining showed CIRP was expressed in the nucleus of neuronal cells,.spermatogonia, primary spermatocytes and epidermal cells, and in the cytoplasm.of the residual tissues. These observations may provide new data to understand.and further study the important role of CIRP protein in the plateau adaptation of.the domestic yak on long-term evolution.