Uterine and placental expression of TRPV6 gene is regulated via progesterone receptor- or estrogen receptor-mediated pathways during pregnancy in rodents
Uterine and placental expression of TRPV6 gene is regulated via progesterone receptor- or estrogen receptor-mediated pathways during pregnancy in rodents
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DOI：
10.1186/1477-7827-7-49
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发表时间：
2009-05-21
影响因子：
4.4
通讯作者：
Jeung, Eui-Bae
中科院分区：
文献类型：
--
作者：
Lee, Bo-Mi;Lee, Geun-Shik;Jeung, Eui-Bae
瞬时受体电位阳离子通道（transient receptor potential cationchannel，subfamily V，member 6，TRPV6）是一种在钙吸收器官中表达的上皮钙通道蛋白。在本研究中，我们研究了啮齿动物妊娠期间子宫和胎盘TRPV6的表达和调节。子宫TRPV6在妊娠第0.5、5.5和13.5天达到高峰，在子宫上皮和腺体中检测到，而胎盘TRPV6 mRNA水平在妊娠中期增加。大鼠子宫和胎盘TRPV6 mRNA水平在妊娠期间出现周期性变化，表明TRPV6可能参与着床过程。此外，子宫TRPV6 mRNA仅在子宫的胎盘未附着区域表达，并且在管腔和腺上皮细胞中观察到子宫TRPV6免疫反应性。在胎盘中，在迷路和海绵区检测到TRPV6。这些结果可能表明TRPV6至少具有两种功能：胚胎植入和维持妊娠。为了研究介导啮齿动物中TRPV6表达的途径，在最大TRPV6表达之前注射抗类固醇激素拮抗剂。在大鼠中，TRPV6表达被RU486（一种抗孕酮剂）通过孕酮受体降低，ICI 182，780（一种抗雌激素剂）通过小鼠中的雌激素受体阻断TRPV6表达。这些组织中表达的子宫和胎盘TRPV6的并列支持TRPV6参与母体和胎儿间室之间的钙离子转移的观点。总之，TRPV6基因可能是控制妊娠期间胚胎和胎盘之间子宫内钙转运的关键元件。
Transient receptor potential cation channel, subfamily V, member 6 (TRPV6) is an epithelial Ca2+ channel protein expressed in calcium absorbing organs. In the present study, we investigated the expression and regulation of uterine and placental TRPV6 during gestation in rodents. Uterine TRPV6 peaked at pregnancy day (P) 0.5, P5.5 and, P13.5 and was detected in uterine epithelium and glands of rats, while placental TRPV6 mRNA levels increased in mid-gestation. Uterine and placental TRPV6 mRNA levels in rats appear to cyclically change during pregnancy, suggesting that TRPV6 may participate in the implantation process. In addition, uterine TRPV6 mRNA is only expressed in placenta-unattached areas of the uterus, and uterine TRPV6 immunoreactivity was observed in luminal and glandular epithelial cells. In the placenta, TRPV6 was detected in the labyrinth and spongy zone. These results may indicate that TRPV6 has at least two functions: implantation of the embryo and maintenance of pregnancy. To investigate the pathway(s) mediating TRPV6 expression in rodents, anti-steroid hormone antagonists were injected prior to maximal TRPV6 expression. In rats, TRPV6 expression was reduced by RU486 (an anti-progesterone) through progesterone receptors, and ICI 182,780 (an anti-estrogen) blocked TRPV6 expression via estrogen receptors in mice. The juxtaposition of uterine and placental TRPV6 expressed in these tissues supports the notion that TRPV6 participates in transferring calcium ions between the maternal and fetal compartments. Taken together, TRPV6 gene may function as a key element in controlling calcium transport in the uterus between the embryo and the placenta during pregnancy.