A better defined medium for human prostate cancer cells.
A better defined medium for human prostate cancer cells.
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一种更明确的人类前列腺癌细胞培养基。
DOI：
10.1007/bf02634145
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Pretlow,TP
中科院分区：
文献类型：
--
作者：
Pretlow,TG;Ogrinc,GS;Amini,SB;Delmoro,CM;Molkentin,KF;Willson,JK;Pretlow,TP
尊敬的编辑：缺乏良好的人前列腺癌（PCA）模型是一个主要的限制，无论是发展的治疗和研究的发病机制，这种肿瘤性疾病。在过去二十年中，许多专家对五氯苯甲醚培养系统的开发做出了重大承诺;然而，全世界只有不到12个五氯苯甲醚细胞系（1、2、4、5、8、14）。只有一个报告（9）在体外转化的人前列腺上皮细胞，这份报告专门涉及新生儿，即青春期前，前列腺细胞。在报告（13）之前，即超过一半的原发性人类PCA在无胸腺小鼠中缓慢生长，直到皮下注射Matrigel中的原发性PCA后3至6个月将动物处死，没有方法允许大多数这些肿瘤在体内或体外增殖超过3个月。Chackal-Roy等人(3)他推断人类PCA经常转移到骨髓腔，并想知道由骨髓或其他类型的细胞产生的生长因子是否可以刺激PCA细胞系的生长。他们测试了PC-3和DU 145 PCA细胞系在1%血清存在下对白细胞介素-1（IL-1）、IL-2、IL-3、IL-4、IL-6、粒细胞（G）-集落刺激因子（CSF）、巨噬细胞（M）-CSF、GM-CSF、表皮生长因子（EGF）、成纤维细胞生长因子（FGF）、血小板衍生生长因子（PDGF）、转化生长因子（TGF）-α、TGF-β、胰岛素、内毒素和骨髓条件培养基（BMCM）。虽然观察到低水平的刺激与几种生长因子，这些因素的反应是小的，与人，大鼠或牛的骨髓细胞条件培养基相比。我们重复了Chackal-Roy等人的工作。(3)稍微修改一下然后，我们检查了由肿瘤性结肠上皮细胞系（15）调节的培养基对这两种PCA细胞系生长的影响。许多条件培养基引起这两种细胞系的刺激与在BMCM中观察到的刺激相当。更重要的是，我们描述了在1%胎牛血清（FCS）存在下，在没有任何其他种类细胞的条件或暴露的情况下，用含有几种确定的补充物的培养基对这两种细胞系的强烈刺激。DU 145不需要血清。PC-3（8）和DU 145（14）细胞系获自美国典型培养物保藏中心（Rockville，MD）。将PC-3细胞维持在补充有7%热灭活FCS（Hyclone Laboratories，Logan，UT）和50 μ g/ml庆大霉素（GIBCO Laboratories）的RPMI 1640培养基（GIBCO Laboratories，Grand Island，NY）中。DU 145细胞在补充有10%热灭活FCS和50 μ g/ml庆大霉素的Eagle最低必需培养基（EMEM）（GIBCO Laboratories）中生长。VACO-330细胞，一种来源于管状息肉的人结肠上皮细胞系（15）在补充有2%FCS、50 μ g/ml庆大霉素、2 mM L-谷氨酰胺（GIBCO Laboratories）、0.1mM非必需氨基酸（GIBCO Laboratories）、10 μ g/ml胰岛素（Sigma Chemical Co.，St. Louis，MO），2 μ g/ml转铁蛋白（Sigma Chemical Co.），1 μ g/ml氢化可的松（Sigma Chemical Co.），和5 × 10-M亚硒酸钠（DIFCO Laboratories，Detroit，MI）。含有最后一句中所列添加剂但不含FCS的培养基将被称为”MEM+"。VACO-330细胞与用作胶原支持物的大鼠尾胶原一起生长。M女士慷慨地捐赠了人骨髓细胞条件培养基。查克罗伊和B博士。泽特中号...
Dear Editor: The lack of good models for human prostatic carcinoma (PCA) is a major limitation both to the development of therapy and for the investigation of the pathogenesis of this neoplastic disease. Many experts have made major commitments to the development of systems for the culture of PCA over the past two decades; however, fewer than a dozen PCA cell lines exist in the entire world (1, 2, 4, 5, 8, 14). There is only one report (9) of transformation in vitro of human prostate epithelial cells, and this report deals exclusively with neonatal, ie, prepubertal, prostate cells. Prior to the report (13) that more than half of primary human PCAs grew slowly in athymic mice until the animals were killed 3 to 6 months after the subcutaneous injection of primary PCA in Matrigel, there was no method that would permit the propagation of most of these tumors in vivo or in vitro for periods in excess of 3 months. Chackal-Roy et al.(3) reasoned that human PCA often metastasizes to bone marrow cavities and wondered if growth factors pro-duced by marrow or by other kinds of cells might stimulate the growth of PCA cell lines. Singly and in combinations of up to three growth factors per culture, they tested the abilities of PC-3 and DU 145 PCA cell lines to respond, in the presence of 1% serum, to interleukin-1 (IL-1), IL-2, IL-3, IL-4, IL-6, granulocyte (G)-colony-stimulating factor (CSF), macrophage (M)-CSF, GM-CSF, epi-dermal growth factor (EGF), fibroblast growth factor (FGF), plate-let-derived growth factor (PDGF), transforming growth factor (TGF)-a, TGF-b, insulin, endotoxin, and bone marrow conditioned medium (BMCM). While low levels of stimulation were observed with several growth factors, the responses to these factors were small as compared with responses to medium conditioned by bone marrow cells from human, rat, or cow. We repeated the work of Chackal-Roy et al.(3) with slight modifications. We then examined the effects of media conditioned by neoplastic colonic epithelial cell lines (15) on the growth of these two PCA cell lines. Many conditioned media caused stimulation of these two cell lines comparable to the stimulation observed in BMCM. More importantly, we de-scribe, in the presence of 1% fetal calf serum (FCS), the vigorous stimulation of these two cell lines by medium with several defined supplements but without any conditioning by or exposure to other kinds of cells. DU 145 required no serum. The PC-3 (8) and DU 145 (14) cell lines were obtained from the American Type Culture Collection (Rockville, MD). PC-3 cells were maintained in RPMI 1640 medium (GIBCO Laboratories, Grand Island, NY) supplemented with 7% heat-inactivated FCS (Hyclone Laboratories, Logan, UT) and 50, g/ml gentamycin (GIBCO Laboratories). DU 145 cells were grown in Eagle's minimum essential medium (EMEM)(GIBCO Laboratories) supplemented with 10% heat-inactivated FCS and 50 jig/ml gentamycin. VACO-330 cells, a line of human colonic epithelial cells derived from a tubular polyp (15) were grown in EMEM supplemented with 2% FCS, 50 Ctg/ml gentamycin, 2 mM L-glutamine (GIBCO Laboratories), 0.1 mM non-essential amino acids (GIBCO Laboratories), 10 ag/ml insulin (Sigma Chemical Co., St. Louis, MO), 2 ttg/ml transferrin (Sigma Chemical Co.), 1 tg/ml hydrocortisone (Sigma Chemical Co.), and5 X 10-M sodium selenite (DIFCO Laboratories, Detroit, MI). Medium with the additives listed in the last sentence, but without FCS, will be termed" MEM+". VACO-330 cells are grown with rat tail collagen used as a collagen support. Medium conditioned by human marrow cells was generously do-nated by Ms. M. Chackal-Roy and Dr. B. Zetter. Medium …