In vitro fungitoxic activity of some glucosinolates and their enzyme-derived products toward plant pathogenic fungi
In vitro fungitoxic activity of some glucosinolates and their enzyme-derived products toward plant pathogenic fungi
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DOI：
10.1021/jf9608635
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发表时间：
1997-07-01
影响因子：
6.1
通讯作者：
Palmieri, S
中科院分区：
文献类型：
--
作者：
Manici, LM;Lazzeri, L;Palmieri, S
芥子油苷是十字花科植物的典型化合物，其酶解产物具有杀菌活性。用黑芥子酶对11种GL及其酶解产物进行了离体抗黄瓜枯萎病菌活性测定。测定了葡萄糖苷、芍药苷、黑芥子苷和表原告依春的水解产物对8种植物病原真菌的毒力。结果表明：天然GLs对真菌没有毒性，但其水解产物对真菌生长有抑制作用，这取决于其化学结构，其中葡萄糖黄苷、葡萄糖芥素、葡萄糖车苷和葡萄糖金莲素的水解产物对真菌生长的抑制作用最强，在0.1mg/mL浓度下抑制率达50%;（iii）从葡伊贝林、葡丹皮酚、黑芥子苷和表原告依春获得的水解产物对八种病原真菌的真菌毒性活性得到证实，根据其化学结构具有不同的响应（iv）最有效的水解产物是来自葡糖苷的那些，显示出对立枯丝核菌，核盘菌，菜豆壳透座菌，最低抑菌浓度为0.1 ~ 1.2mg/mL。
The enzymatic hydrolysis of glucosinolates (GLs), typical compounds of Cruciferae, produces molecules with fungitoxic activity. Eleven GLs and their enzymatic hydrolysis products obtained by myrosinase were tested in vitro against Fusarium culmorum. Toxicity of hydrolysis products from glucoiberin, glucotropaeolin, sinigrin, and epiprogoitrin were assayed on eight plant pathogenic fungi. The results showed (i) the native GLs showed no fungitoxic activity, whereas their hydrolysis products inhibited fungal growth depending on their chemical structure; (ii) the hydrolysis products from glucoiberin, glucoerucin, glucocheirolin, and glucotropaeolin were the most effective, with 50% inhibition of fungal growth at 0.1 mg/mL; (iii) the fungitoxic activity of hydrolysis products obtained from glucoiberin, glucotropaeolin, sinigrin, and epiprogoitrin was confirmed on eight pathogenic fungi, with different responses depending on their chemical structure (iv) the most effective hydrolysis products were those from glucoiberin, showing EC50 values of 0.05 mg/mL on Rhizoctonia solani, Sclerotinia sclerotiorum, Diaporthe phaseolorum, and Pythium irregulare and a minimum inhibitory concentration varying from 0.1 to 1.2 mg/mL.