Andean berry (Vaccinium meridionale Swartz) juice in combination with Aspirin modulated anti-inflammatory markers on LPS-stimulated RAW 264.7 macrophages
Andean berry (Vaccinium meridionale Swartz) juice in combination with Aspirin modulated anti-inflammatory markers on LPS-stimulated RAW 264.7 macrophages
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DOI：
10.1016/j.foodres.2020.109541
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发表时间：
2020-11-01
影响因子：
8.1
通讯作者：
Campos-Vega, Rocio
中科院分区：
文献类型：
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作者：
Arango-Varela, Sandra S.;Luzardo-Ocampo, Ivan;Campos-Vega, Rocio
V. Swartz是一种未被充分利用的安第斯浆果，与其抗炎作用可能产生的几种健康益处有关。本研究旨在评估安第斯浆果汁（ABJ）联合阿司匹林对LPS刺激的RAW 264.7巨噬细胞抗炎标志物的调节作用。ABJ的化学特征表明多酚类物质含量较高，主要是没食子酸（659-75 μ g/g）和氯化花青素（418.61 μ g/mL）。与LPS刺激的巨噬细胞相比，ABJ、阿司匹林及其组合降低了NO和ROS的产生（分别为3.26- 42.55%和17.59- 65.68%）。相比之下，发现NO还原的半抑制浓度（IC 50）为7.69%v/v（ABJ）和24.48mM（阿司匹林）。与促炎对照（LPS）相比，ABJ降低了IL-1 β、MCP-1和GCSF;阿司匹林降低了IL 1 R 1、MCP-1、GMCSF、GCSF和TNF-α; ABJ +阿司匹林治疗降低了IL 1 R、GMCSF和CXCL 10。细胞因子的计算机相互作用和潜在结合相互作用的预测表明CCR 1、CCR 5和NF-κ B调节。这些结果显示了未被充分利用的南美浆果的抗炎潜力及其与已知药物如阿司匹林在解决炎症性疾病中的协同辅助作用。这是第一份关于V. Escherionale Swartz果汁与阿司匹林联合对LPS攻击的RAW 264.7巨噬细胞的抗炎作用的报告。
V. meridionale Swartz is an underutilized Andean Berry that has been linked to several health benefits potentially derived from its anti-inflammatory effects. This research aimed to evaluate the impact of Andean Berry Juice (ABJ) combined with Aspirin in the modulation of anti-inflammatory markers from LPS-stimulated RAW 264.7 macrophages. The chemical characterization of ABJ showed a high content of polyphenols, mainly gallic acid (659-75 mu g/g) and cyanidin chloride (418.61 mu g/mL). Compared to LPS-stimulated macrophages, ABJ, Aspirin, and its combination reduced NO and ROS production (3.26-42.55 and 17.59-65.68%, respectively). In comparison, the half inhibitory concentration of NO reduction (IC50) was found at 7.69% v/v (ABJ) and 24.48 mM (Aspirin). Compared to the pro-inflammatory control (LPS), ABJ reduced IL-1 beta, MCP-1, and GCSF; Aspirin decreased IL1R1, MCP-1, GMCSF, GCSF, and TNF-alpha; and the ABJ + Aspirin treatment reduced IL1R, GMCSF, and CXCL10. The in silico interaction of cytokines and the prediction of potential binding interactions suggested CCR1, CCR5, and NF-kB modulation. These results showed the anti-inflammatory potential of underutilized South American berries and their co-adjuvant effect with known drugs such as Aspirin in the resolution of inflammatory-derived conditions. This is the first report of the anti-inflammatory effects of V. meridionale Swartz juice in combination with Aspirin on LPS-challenged RAW 264.7 macrophages.