1 alpha,25-(OH)2 vitamin D3 enhances expression of the genes encoding Ca(2+)-binding proteins MRP-8 and MRP-14.
1 alpha,25-(OH)2 vitamin D3 enhances expression of the genes encoding Ca(2+)-binding proteins MRP-8 and MRP-14.
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1 α,25-(OH)2 维生素 D3 增强编码 Ca(2) 结合蛋白 MRP-8 和 MRP-14 的基因表达。
DOI：
10.1152/ajpcell.1992.262.1.c235
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Bonventre,JV
中科院分区：
文献类型：
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作者：
Sellmayer,A;Krane,SM;Ouellette,AJ;Bonventre,JV
两种密切相关的Ca（2+）结合蛋白，迁移抑制因子相关蛋白（MRP）-8和MRP-14，是在骨髓细胞分化的特定条件下合成的。由于1 α，25-二羟维生素D3 [1，25-（OH）2D 3]诱导髓样细胞分化和其他S-100类钙结合蛋白的表达，我们研究了1，25-（OH）2D 3对人U-937组织细胞淋巴瘤细胞中MRP mRNA水平的影响。1，25-（OH）_2D_3以时间和剂量依赖的方式增加MRP-8和MRP-14 mRNA的表达。MRP mRNA水平在24小时达到最大值，并在细胞暴露于1，25-（OH）2D 3后至少96小时保持升高。MRP-8 mRNA的积累需要100- 1，000倍高浓度的25-（OH）D3，其与1，25-（OH）2D 3细胞内受体结合的亲和力低100- 1，000倍。其他分化剂，二甲亚砜，维甲酸，地塞米松，也增加了MRP-8和MRP-14 mRNA的水平。佛波醇肉豆蔻酸酯增强MRP-14 mRNA水平的程度大于MRP-8 mRNA水平，表明蛋白激酶C对MRP基因表达的差异调节。1，25-（OH）2D 3诱导的MRP mRNA水平的相对增加并不因细胞外[Ca 2 +]减少1，000倍而改变。因此，1，25-（OH）2D 3可能是MRP基因表达的生理调节剂。MRP-8和MRP-14基因的表达可能对骨髓细胞的分化很重要。
Two closely related Ca(2+)-binding proteins, migration inhibitory factor-related protein (MRP)-8 and MRP-14, are synthesized under specific conditions of myeloid cell differentiation. Because 1 alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] induces myeloid cell differentiation and expression of other S-100 class calcium-binding proteins, we examined the effects of 1,25-(OH)2D3 on MRP mRNA levels in human U-937 histiocytic lymphoma cells. 1,25-(OH)2D3 increased MRP-8 and MRP-14 mRNA levels in a time- and dose-dependent manner. MRP mRNA levels were maximal at 24 h and remained elevated for at least 96 h after exposure of the cells to 1,25-(OH)2D3. MRP-8 mRNA accumulation required 100- to 1,000-fold higher concentrations of 25-(OH)D3, which binds to the 1,25-(OH)2D3 intracellular receptor with 100- to 1,000-fold lower affinity. Other differentiating agents, dimethyl sulfoxide, retinoic acid, and dexamethasone, also increased levels of MRP-8 and MRP-14 mRNA. Phorbol myristate acetate enhanced MRP-14 mRNA levels to a greater extent than MRP-8 mRNA levels, suggesting differential regulation of MRP gene expression by protein kinase C. The 1,25-(OH)2D3-induced relative increase in MRP mRNA levels was not changed by a 1,000-fold reduction in extracellular [Ca2+]. Thus 1,25-(OH)2D3 is potentially a physiological modulator of MRP gene expression. Expression of the MRP-8 and MRP-14 genes may be important for differentiation of myeloid cells.