Quantitative microscopy-based measurements of circulating nanoparticle concentration using microliter blood volumes.
Quantitative microscopy-based measurements of circulating nanoparticle concentration using microliter blood volumes.
复制标题
使用微升血容量进行基于定量显微镜的循环纳米颗粒浓度测量。
DOI：
10.1016/j.nano.2017.04.003
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Saltzman,WMark
中科院分区：
文献类型：
--
作者：
Tietjen,GregoryT;DiRito,Jenna;Pober,JordanS;Saltzman,WMark
Nanoparticles (NPs) are potential drug delivery vehicles for treatment of a broad range of diseases. Intravenous (IV) administration, the most common form of delivery, is relatively non-invasive and provides (in theory) access throughout the circulatory system. However, in practice, many IV injected NPs are quickly eliminated by specialized phagocytes in the liver and spleen. Consequently, new materials have been developed with the capacity to significantly extend the circulating half-life of IV administered NPs. Unfortunately, current procedures for measuring circulation half-lives are often expensive, time consuming, and can require large blood volumes that are not compatible with mouse models of disease. Here we describe a simple and reliable procedure for measuring circulation half-life utilizing quantitative microscopy. This method requires only 2 μL of blood and minimal sample preparation, yet provides robust quantitative results.
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