Circulating myeloid dendritic cells are increased in individuals with severe aplastic anemia
Circulating myeloid dendritic cells are increased in individuals with severe aplastic anemia
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严重再生障碍性贫血患者的循环骨髓树突状细胞增加
DOI：
10.1007/s12185-010-0761-z
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发表时间：
2011-02-01
影响因子：
2.1
通讯作者：
Wu Yuhong
中科院分区：
文献类型：
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作者：
Shao Zonghong;Tu Meifeng;Wu Yuhong
本研究的目的是研究强化免疫抑制治疗（IST）前后严重再生障碍性贫血（SAA）患者外周血单个核细胞（PBMC）中髓样树突状细胞（mDC）和浆细胞样树突状细胞（pDC）的数量，并评估SAA患者树突状细胞（DC）表达的共刺激分子（CD80、CD86和CD40）的表达。对38例SAA活跃期患者、19例恢复期患者和17例正常对照患者的PBMC中mDC和pDC的数量及mDC与pDC的比值进行了测定。分析16例SAA患者和15例正常人dc和B淋巴细胞表面CD80、CD86和CD40的表达情况。活动期SAA患者的mDC百分比和mDC:pDC比值较健康对照组升高[分别为0.65%（0.10 ~ 2.19%）比0.40%（0.11 ~ 1.54%）、2.64%（1.07 ~ 4.33%）比1.56%(0.89 ~ 2.27)，差异均有统计学意义（P< 0.05）]。恢复SAA患者中mDC的百分比下降到0.43%（范围0.06 ~ 0.80），mDC:pDC的比例下降到1.78%（范围0.49 ~ 3.07）。10例SAA患者的mDC和pDC百分比在IST前分别为0.87%（0.10 ~ 1.85）和0.35% (0.05 ~ 0.65)，IST后分别为0.24%（0.06 ~ 0.52）和0.14% (0.01 ~ 0.28)（P< 0.05）。与健康对照组相比，SAA患者DC上CD86的表达比例分别为29.84%（20.28 ~ 39.40）和11.97%(0.02 ~ 24.15)，差异有统计学意义（P< 0.05）。SAA患者的mDCs数量增加，这与疾病分期有关。mDCs数量的增加和这些dc上共刺激分子（CD86）的高表达可能导致这些患者T淋巴细胞的异常活化和随后的免疫系统介导的骨髓衰竭。
The objectives of the study were to investigate the number of myeloid dendritic cells (mDC) and plasmacytoid dendritic cells (pDC) present in peripheral blood mononuclear cells (PBMC) from severe aplastic anemia (SAA) patients before and after intensive immunosuppressive therapy (IST) and to assess the expression of co-stimulatory molecules (CD80, CD86, and CD40) expressed by dendritic cells (DC) from SAA patients. The quantities of mDC and pDC and ratios of mDC to pDC in PBMC were measured in 38 SAA patients at active phase, 19 patients at recovery phase, and 17 normal controls. The surface expression of CD80, CD86, and CD40 on DCs and B lymphocytes was analyzed in 16 SAA patients and 15 normal controls. The percentages of mDC and the ratio of mDC:pDC of SAA patients at active phase increased compared to that of healthy controls [0.65% (range 0.10–2.19%) vs. 0.40% (range 0.11–1.54%), 2.64% (range 1.07–4.33%) vs. 1.56% (range 0.89–2.27), respectively (P< 0.05)]. The percentages of mDCs in recovered SAA patients decreased to 0.43% (range 0.06–0.80), and the ratio of mDC:pDC decreased to 1.78% (range 0.49–3.07). The percentages of mDC and pDC in 10 SAA patients were 0.87% (range 0.10–1.85) and 0.35% (range 0.05–0.65) before IST, which decreased to 0.24% (range 0.06–0.52) and 0.14% (range 0.01–0.28) after IST (P< 0.05). The percentages of CD86 expression on DC of SAA patients increased compared to that of healthy controls [29.84% (range 20.28–39.40) vs. 11.97% (range 0.02–24.15), respectively (P< 0.05)]. The number of mDCs increased in SAA patients, which was associated with stage of disease. The increased number of mDCs and the high expression of costimulatory molecules (CD86) on these DCs may contribute to abnormal activation of T lymphocytes in these patients and subsequent immune system-mediated bone marrow failure.