Langmuir monolayer properties of the fluorinated-hydrogenated hybrid amphiphiles with dipalmitoylphosphatidylcholine (DPPC).
Langmuir monolayer properties of the fluorinated-hydrogenated hybrid amphiphiles with dipalmitoylphosphatidylcholine (DPPC).
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DOI：
10.1016/j.colsurfb.2005.12.006
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发表时间：
2006-02
期刊：
影响因子：
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通讯作者：
K. Hoda;H. Nakahara;Shohei Nakamura;S. Nagadome;G. Sugihara;N. Yoshino;O. Shibata
中科院分区：
文献类型：
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作者：
K. Hoda;H. Nakahara;Shohei Nakamura;S. Nagadome;G. Sugihara;N. Yoshino;O. Shibata
获得了两种氟化氢化杂化两亲分子的Langmuir单分子膜的表面压-面积（π-A）、表面势-面积（ΔV-A）和偶极矩-面积（μ V-A）等温线（1-[（4-全氟己基）-苯基]-1-己基磷酸苯酯钠（F6 PH 5 PPhNa）和（1-[（4-全氟辛基）-苯基]-1-己基磷酸苯基钠（F8 PH 5 PPhNa））、DPPC及其在空气/水界面上的双组分体系。用Wilhelmy法、离子化电极法和荧光显微镜法研究了298.2K下在0.02MTris缓冲溶液（pH7.4）和0.13MNaCl溶液中形成的单分子膜。此外，两个组分的相容性进行了检查，通过绘制的分子面积和表面电位的变化作为氟化-氢化的混合两亲物的摩尔分数的基础上的加和性规则的函数。的monlayers的结晶性也检查通过构建二维相图。此外，假设规则的表面混合物，用于分析双组分单分子膜的坍塌压力的Joos方程允许计算氟化-氢化混合两亲物与DPPC之间的相互作用参数（ε）和相互作用能（-Δ ε）。通过荧光显微镜的观察也支持我们的解释，作为在单层状态的可折叠性。比较两个二元体系的单分子层行为，发现在各个方面没有显著差异。在这两种组合中，单层性质的摩尔分数依赖性通常分为两个范围：0≤X≤0.3和0.3 ≤ X≤1。分子堆积和表面势反映了含氟部分链长的依赖性。
Surface pressure–area (π–A), surface potential–area (ΔV–A), and dipole moment–area (μ⊥–A) isotherms were obtained for the Langmuir monolayer of two fluorinated-hydrogenated hybrid amphiphiles (sodium phenyl 1-[(4-perfluorohexyl)-phenyl]-1-hexylphosphate (F6PH5PPhNa) and (sodium phenyl 1-[(4-perfluorooctyl)-phenyl]-1-hexylphosphate (F8PH5PPhNa)), DPPC and their two-component systems at the air/water interface. Monolayers spread on 0.02M Tris buffer solution (pH 7.4) with 0.13M NaCl at 298.2K were investigated by the Wilhelmy method, ionizing electrode method and fluorescence microscopy. Moreover, the miscibility of two components was examined by plotting the variation of the molecular area and the surface potential as a function of the molar fraction for the fluorinated-hydrogenated hybrid amphiphiles on the basis of the additivity rule. The miscibility of the monlayers was also examined by construction of two-dimensional phase diagrams. Furthermore, assuming the regular surface mixture, the Joos equation for analysis of the collapse pressure of two-component monolayers allowed calculation of the interaction parameter (ξ) and the interaction energy (−Δɛ) between the fluorinated-hydrogenated hybrid amphiphiles and DPPC. The observations by a fluorescence microscopy also supported our interpretation as for the miscibility in the monolayer state. Comparing the monolayer behavior between the two binary systems, no remarkable difference was found among various aspects. Among the two combinations, the mole fraction dependence in monlayer properties was commonly classified into two ranges: 0≤X≤0.3 and 0.3<X≤1. Dependence of the chain length of fluorinated part was reflected for the molecular packing and surface potential.