pH-Activatable Cyanine Dyes for Selective Tumor Imaging Using Near-Infrared Fluorescence and Photoacoustic Modalities
pH-Activatable Cyanine Dyes for Selective Tumor Imaging Using Near-Infrared Fluorescence and Photoacoustic Modalities
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DOI：
10.1021/acssensors.0c01926
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发表时间：
2021-01-22
期刊：
影响因子：
8.9
通讯作者：
Ohe, Kouichi
中科院分区：
文献类型：
--
作者：
Mu, Huiying;Miki, Koji;Ohe, Kouichi
光声（PA）成像是一种新兴的分子成像模式，补充荧光成像，并实现深层组织内的高分辨率。荧光/PA多模态成像将是一种强大的技术，以提取更全面的信息，从目标比传统的单模态成像。在本文中，我们开发了一种新的pH激活传感器，CypHRGD，这是适用于荧光和PA检测。CypHRGD源自我们以前的近红外pH传感平台，其中用大体积苯基取代并用cRGD肽官能化显著改善了传感器的生物相容性，减弱了染料聚集。CypHRGD的多模态成像应用在培养的细胞和癌症异种移植小鼠中被证明具有快速动力学和高灵敏度和特异性，这依赖于cRGD促进的肿瘤靶向，探针积累和随后的内吞作用后酸性细胞器中的激活。
Photoacoustic (PA) imaging is an emerging molecular imaging modality that complements fluorescence imaging and enables high resolution within deep tissue. Fluorescence/PA multimodality imaging would be a powerful technique to extract more comprehensive information from targets than traditional single-modality imaging. In this paper, we developed a new pH-activatable sensor, CypHRGD, which is applicable to both fluorescence and PA detection. CypHRGD was derived from our previous near-infrared pH-sensing platform, in which substitution with a bulky phenyl group and functionalization with a cRGD peptide remarkably improved the sensor's biocompatibility with attenuated dye aggregation. The multi-modality imaging applications of CypHRGD were demonstrated in cultured cells and cancer-xenografted mice with rapid kinetics and high sensitivity and specificity, which relies on cRGD-facilitated tumor targeting, probe accumulation and subsequent activation in the acidic organelles after endocytosis.