Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo
Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo
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体内细菌鞭毛运动蛋白之间的定点交联
DOI：
10.1007/978-1-0716-3060-0_7
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发表时间：
2023
影响因子：
--
通讯作者：
Kojima Seiji
中科院分区：
文献类型：
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作者：
Terashima Hiroyuki;Homma Michio;Kojima Seiji
细菌鞭毛采用嵌入细胞表面的旋转电机。电机由定子和转子元件组成，并由通过定子离子通道的离子流入（通常为H+或Na+）驱动。离子流入诱导定子中的构象变化，随后是定子和转子之间的相互作用的变化。旋转鞭毛的驱动力被认为是通过改变定子-转子相互作用产生的。在这一章中，我们描述了两种方法来研究定子和转子之间的相互作用：定点体内光交联和定点体内半胱氨酸二硫键交联。
The bacterial flagellum employs a rotary motor embedded on the cell surface. The motor consists of the stator and rotor elements and is driven by ion influx (typically H+or Na+) through an ion channel of the stator. Ion influx induces conformational changes in the stator, followed by changes in the interactions between the stator and rotor. The driving force to rotate the flagellum is thought to be generated by changing the stator-rotor interactions. In this chapter, we describe two methods for investigating the interactions between the stator and rotor: site-directed in vivo photo-crosslinking and site-directed in vivo cysteine disulfide crosslinking.