Virus isolation and quantitation
Virus isolation and quantitation
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DOI：
10.1016/b978-012465330-6/50003-8
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Killington RA
中科院分区：
文献类型：
--
作者：
Hierholzer JC;Killington RA
本章概述了两种最常用的病毒分离方法--即组织培养和胚胎卵子。本章描述了涵盖临床情况中遇到的大多数病毒的主要病毒组所涉及的方法。本章还介绍了选定的病毒检测方法和病毒滴度的计算。鼻拭子是最容易收集的呼吸道病毒样本，也是本章所述大多数呼吸道病毒的最佳样本。血吸法是一种快速、简便的检测细胞培养中正粘病毒和非呼吸道合胞病毒副粘病毒的方法，其细胞病变效应从明显到微小不等。
This chapter outlines the two most commonly used methods of virus isolation—namely, tissue cultures and embryonated eggs. The chapter describes the methodology involved for the predominant virus groups that cover the majority of viruses encountered in the clinical situation. The chapter also describes selected methods of virus assay and the calculation of virus titers. Nasal swabs are the easiest specimens to collect for respiratory viruses and are also the best specimens for the majority of the respiratory viruses described in the chapter. Hemadsorption is a fast and convenient method of detecting orthomyxoviruses and non respiratory syncytial virus paramyxoviruses in cell cultures in which the cytopathic effect vary from obvious to minimal.