Delineation of the functional capacity of human neonatal lymphocytes.
Delineation of the functional capacity of human neonatal lymphocytes.
复制标题
人类新生儿淋巴细胞功能的描述。
DOI：
10.1172/jci115029
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Lipsky,PE
中科院分区：
文献类型：
--
作者：
Splawski,JB;Jelinek,DF;Lipsky,PE
利用T细胞依赖的多克隆B细胞活化和IG分泌系统研究了新生儿T细胞-B细胞协作。在该系统中，由固定的抗-CD 3激活的T细胞为成人淋巴细胞产生IG提供了有力的刺激。相比之下，抗CD 3刺激脐带血淋巴细胞产生的免疫球蛋白分泌细胞的数量最少。新生儿淋巴细胞产生的IG增加了金黄色葡萄球菌或分泌因子从有丝分裂原刺激的成人T细胞。补充IL-2导致产生大量IgM和少量IgG和伊加，产生的IG少于成人淋巴细胞的可比培养物。新生儿T细胞增殖并产生IL-2以响应固定的抗-CD 3，并支持B细胞增殖和成人B细胞的IG分泌，尽管不如成人T细胞有效。来自活化的新生儿T细胞的上清液在其支持成人B细胞产生IG的能力方面明显受限。新生儿B细胞可被诱导分化，以响应抗CD 3刺激的成人T细胞。然而，分泌的IgG和伊加的量与成人水平相比很小。这些研究表明新生儿B细胞和T细胞存在相对而非绝对的功能缺陷。图片
Neonatal T cell-B cell collaboration was investigated utilizing a system of T cell-dependent polyclonal B cell activation and Ig secretion. In this system, T cells activated by immobilized anti-CD3 provide a potent stimulus for Ig production by adult lymphocytes. By contrast, anti-CD3 stimulation of cord blood lymphocytes generated minimal numbers of Ig-secreting cells. Ig production by neonatal lymphocytes was enhanced by the addition of Staphylococcus aureus or secreted factors from mitogen-stimulated adult T cells. Supplementation with IL-2 resulted in the production of large amounts of IgM and small amounts of IgG and IgA, with less Ig produced than by comparable cultures of adult lymphocytes. Neonatal T cells proliferated and produced IL-2 in response to immobilized anti-CD3, and supported B cell proliferation and Ig secretion by adult B cells, although not as effectively as adult T cells. Supernatants from activated neonatal T cells were markedly limited in their capacity to support Ig production by adult B cells. Neonatal B cells could be induced to differentiate in response to anti-CD3-stimulated adult T cells. However, the amounts of IgG and IgA secreted were small compared to adult levels. These studies indicate a relative, but not absolute, functional deficiency of both neonatal B and T cells.Images