GROWTH OF ADULT-RAT RETINAL GANGLION-CELL NEURITES ON ASTROCYTES
GROWTH OF ADULT-RAT RETINAL GANGLION-CELL NEURITES ON ASTROCYTES
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DOI：
10.1002/glia.440030409
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发表时间：
1990-01-01
期刊：
影响因子：
6.2
通讯作者：
BUNGE, RP
中科院分区：
文献类型：
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作者：
BAEHR, M;BUNGE, RP
星形胶质细胞，以及雪旺细胞（SC），可以在发育过程中为胚胎神经突提供合适的基质，但它们支持成体再生神经突的能力尚未直接比较。本研究的目的是确定星形胶质细胞促进成年大鼠视网膜神经节细胞（RGC）在体外再生的能力，并将其与先前确定的雪旺细胞表面生长进行比较。我们制备了I型星形胶质细胞（Raff等人：J. Neurosci. 3：1289-1300，1983）。将这些细胞在无血清培养基中传代培养并维持至少2周（对层粘连蛋白几乎没有免疫反应性的星形胶质细胞）或在含血清培养基中传代培养7至10天（对层粘连蛋白具有免疫反应性的扁平和多边形星形胶质细胞）。因此，星状星形胶质细胞可能代表体内成熟的星形胶质细胞（Ard和Bunge：J. Neurosci. 8：2844-2858，1988），而扁平星形胶质细胞可能类似于未成熟的脑星形胶质细胞（Liesi等人：J. Cell Biol.96：920-924，1983）。将这些神经胶质细胞群上的成年RGC存活和轴突再生长与在不同SC群上观察到的进行比较，如先前报道的（Baehr和Bunge：Exp. Neurol. 106：27-40，1989）。两种星形胶质细胞群体（扁平或星形胶质细胞）均未增强RGC存活。星形胶质细胞在支持RGC轴突再生方面不如扁平星形胶质细胞有效。当将这些数据与SC上的RGC存活和轴突生长进行比较时（Baehr和Bunge：Exp. Neurol. 106：27-40，1989）在增强RGC存活和神经突再生长方面，仅“活化的”成熟SC群上级星形胶质细胞。这些结果表明：1）星形胶质细胞和“未成熟”SC在支持RGC存活的能力方面相似; 2）“活化的”成熟SC群体在促进RGC存活和神经突生长方面明显优于星形胶质细胞和“未成熟”SC; 3）星状（“成熟”）星形胶质细胞尽管允许轴突再生，但并不是再生成人RGC神经突的有利基质，也不能有效地支持研资局的生存。
Astrocytes, as well as Schwann cells (SC), can provide suitable substrata for embryonic neurites during development, but their abilities to support adult regenerating neurites have not been directly compared. The aim of the present study was to determine the ability of astrocytes to promote adult rat retinal ganglion cell (RGC) regeneration in vitro and to compare this to previously determined growth on the surface of Schwann cells. We prepared Type I astrocytes (Raff et al: J. Neurosci. 3: 1289–1300, 1983) from perinatal rats. These were subcultured and maintained in either a serum-free medium for at least 2 weeks (stellate astrocytes with little immunoreactivity for laminin) or in serum containing medium for 7 to 10 days (flat and polygonal astrocytes with immunoreactivity for laminin). Stellate astrocytes might therefore represent mature astrocytes in vivo (Ard and Bunge: J. Neurosci. 8: 2844–2858, 1988), while flat astrocytes might resemble immature brain astrocytes (Liesi et al: J. Cell Biol. 96: 920–924, 1983). Adult RGC survival and axonal regrowth on these glia populations was compared to that observed on different SC populations, as previously reported (Baehr and Bunge: Exp. Neurol. 106: 27–40, 1989). Both astrocyte populations (either flat or stellate astrocytes) did not enhance RGC survival. Stellate astrocytes were less effective in supporting RGC axon regeneration than flat astrocytes. When these date were compared to RGC survival and axon growth on SC (Baehr and Bunge: Exp. Neurol. 106: 27–40, 1989) only “ctivated” mature SC populations were superior to astrocytes in enhancing RGC survival and neurite regrowth. These results suggest 1) that astrocytes and “immature” SC are similar in their ability to support RGC survival; 2)“activated” mature SC populations are significantly better than astrocytes and “immature” SC in enhancing RGC survival and neurite growth; 3) stellate (“mature”) astrocytes, although permissive for regrowing axons, are not a favorable substrate for regenerating adult RGC neurites, nor do they effectively support RGC survival.