The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats
The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats
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DOI：
10.1093/jn/133.1.21
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发表时间：
2003-01-01
影响因子：
4.2
通讯作者：
Lecannu, G
中科院分区：
文献类型：
--
作者：
Cherbut, C;Michel, C;Lecannu, G
低聚果糖（FOS）能促进乳酸菌（LAB）生长，促进丁酸和乳酸的产生。由于这些特性，FOS可能有益于肠道炎症。本研究的目的是研究FOS对大鼠结肠炎的影响，并确定哪些因素参与其中。结肠内三硝基苯磺酸（TNBS）诱导的结肠炎大鼠组接受9 g/L NaCl、1 g/d FOS或10（11）菌落形成单位（cfu）/d LAB的胃内输注（实验1），或结肠内输注9 g/L NaCl、丁酸盐、乳酸盐或丁酸盐+乳酸盐（含或不含10（9.5）cfu/d LAB）（实验2）。每次输注2次/d，共14 d。胃内FOS可降低盲肠的炎症评分（P < 0.001）、髓过氧化物酶（MPO）活性（P < 0.001）和pH值（P < 0.001），增加乳酸（P = 0.02）和丁酸（P < 0.001）以及乳酸菌计数（P < 0.01）。胃内乳酸菌（10（11）cfu/d）具有与FOS相同的有益效果，并类似地改变盲肠组成。结肠内给予高剂量丁酸盐和乳酸盐可降低炎症指数（P < 0.001），而结肠内给予低浓度丁酸盐和乳酸盐则倾向于降低炎症和MPO活性的总评分（P < 0.1）。在有机酸中添加LAB（10（9.5）cfu/d）是重现福斯对这些变量的显著诱导效应所必需的。因此，在所用的实验条件下，FOS主要通过增加肠道中的LAB计数来减少肠道炎症活动。
Fructooligosaccharides (FOS) increase the growth of lactic acid bacteria (LAB) and promote butyrate and lactate production. Because of these properties, FOS may benefit intestinal inflammation. The purpose of this study was to investigate the effect of FOS on colitis in rats and determine which factors are involved. Groups of rats with intracolonic trinitrobenzene sulfonic acid (TNBS)-induced colitis received intragastric infusions of 9 g/L NaCl, 1 g/d FOS or 10(11) colony-forming units (cfu)/d LAB (Experiment 1), or intracolonic infusions of 9 g/L NaCl, butyrate, lactate or butyrate + lactate with or without 10(9.5) cfu/d LAB (Experiment 2). Each infusion was administered twice daily for 14 d. Intragastric FOS reduced the gross score for inflammation (P < 0.001), myeloperoxidase (MPO) activity (P < 0.001) and pH (P < 0.001), and increased lactate (P = 0.02) and butyrate concentrations (P < 0.001) as well as LAB counts in the cecum (P < 0.01). Intragastric LAB (10(11) cfu/d) had the same beneficial effects as FOS and modified the cecal composition similarly. High doses of intracolonic butyrate and lactate reduced the indices of inflammation (P < 0.001), whereas administration of the lower concentrations found in the colon tended to decrease (P < 0.1) the gross score for inflammation and MPO activity. Addition of LAB (10(9.5) cfu/d) to the organic acids was necessary to reproduce the significant FOS-induced effects on these variables. Thus, under the experimental conditions used, FOS reduced intestinal inflammatory activity mainly by increasing LAB counts in the intestine.