An atomic force microscopic examination of the apical membrane of the mammalian gastric gland.
An atomic force microscopic examination of the apical membrane of the mammalian gastric gland.
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哺乳动物胃腺顶膜的原子力显微镜检查。
DOI：
10.1006/cbir.1997.0217
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Geibel,J
中科院分区：
文献类型：
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作者：
Geibel,J
作者用原子力显微镜观察了大鼠和家兔活体胃腺顶膜的形态，采用传统的直立构型和新的倒置生物显微镜模式。用手解剖个别胃腺，并用显微外科方法暴露其根尖膜。裂开的腺体允许进入并直接成像4-6个细胞，原子力显微镜的扫描尖端可以看到它们的根尖轴。所有细胞在生理林格液中37°C扫描。扫描显示，我们可以看到顶细胞和主细胞，并通过其独特的顶端地形来区分它们。壁细胞沿细胞顶轴分布有多种小管。主要细胞的扫描显示顶端表面沿整个顶端边缘被微绒毛覆盖。这些研究结果表明，在37°C下对活体胃腺进行成像以及观察壁细胞和主细胞的表面拓扑结构确实是可行的。
The authors have examined the morphology of the apical membrane of living gastric glands from both the rat and rabbit with an atomic force microscope using both a conventional upright configuration and in the new inverted bioscope mode. Individual gastric glands were hand dissected and the apical membrane was exposed using a microsurgical approach. The split open glands allowed to access and directly image 4–6 cells with their apical axis available to the scanning tip of the atomic force microscope. All cells were scanned in a physiological Ringer solution at 37°C. The scans revealed that we could visualize both parietal and chief cells and to distinguish them via their unique apical topography. The parietal cells showed a variety of small canaliculi that were dispersed along the apical axis of the cell. Scans of chief cells revealed an apical surface that was covered with microvilli along the entire apical margin. The results of these studies show that it is indeed feasible to image living gastric glands at 37°C and to observe the surface topology of both the parietal and chief cell.