ACETYLATED HISTONE H-4 IS PREFERENTIALLY ASSOCIATED WITH TEMPLATE-ACTIVE CHROMATIN
ACETYLATED HISTONE H-4 IS PREFERENTIALLY ASSOCIATED WITH TEMPLATE-ACTIVE CHROMATIN
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DOI：
10.1073/pnas.75.8.3574
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
CANDIDO, EPM
中科院分区：
文献类型：
--
作者：
DAVIE, JR;CANDIDO, EPM
用DNase II（脱氧核糖核酸3“-谷胱甘肽水解酶; EC 3.1.4.6）消化来自发育早期的鳟鱼睾丸的染色质，并将溶解的产物分级分离成Mg 2+可溶性和Mg 2+不溶性组分。1-和2-维聚丙烯酰胺凝胶从这些馏分的组蛋白的检查表明，高度乙酰化的组蛋白H4（二，三，四乙酰化）的物种主要与Mg 2 +-可溶性材料。用胰腺核糖核酸酶消化该染色质部分，将其一半以上转化为不溶性状态，乙酰化的H4保持与沉淀部分相关联。未观察到其他组蛋白的变化，但其他2种碱性蛋白与Mg 2+可溶性组分相关。由于该部分富含转录基因序列，因此活性基因的组蛋白H4可能以高度乙酰化的状态存在。
Chromatin from trout testis at an early stage of development was digested with DNase II (deoxyribonucleate 3''-oligonucleotidohydrolase; EC 3.1.4.6), and the solubilized products were fractionated into Mg2+-soluble and -insoluble components. An examination of the histones from these fractions by 1- and 2-dimensional polyacrylamide gels showed that the highly acetylated species of histone H4 (di-, tri-, and tetra-acetylated) were associated mainly with the Mg2+-soluble material. Digestion of this chromatin fraction with pancreatic RNase converted more than half of it to an insoluble state, and the acetylated H4 remained associated with the precipitated fraction. No changes in the other histones were noted, but 2 other basic proteins were associated with the Mg2+-soluble fraction. Since this fraction is enriched in transcribing gene sequences, it may be that the histone H4 of active genes is present in a highly acetylated state.