CALCIUM MOBILIZATION AND EXOCYTOSIS AFTER ONE MECHANICAL STRETCH OF LUNG EPITHELIAL-CELLS
CALCIUM MOBILIZATION AND EXOCYTOSIS AFTER ONE MECHANICAL STRETCH OF LUNG EPITHELIAL-CELLS
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DOI：
10.1126/science.2173861
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发表时间：
1990-11-30
期刊：
影响因子：
56.9
通讯作者：
DOBBS, LG
中科院分区：
文献类型：
--
作者：
WIRTZ, HRW;DOBBS, LG
肺的深度膨胀刺激表面活性剂的分泌机制尚不清楚。通过拉伸硅橡胶膜上培养的II型细胞，验证了机械膨胀直接刺激II型细胞分泌表面活性剂的假设。在单细胞中，拉伸前后测量细胞内Ca2+浓度。肺泡II型细胞的单次拉伸引起细胞质Ca2+的短暂（不到60秒）增加，随后持续（15至30分钟）刺激表面活性剂分泌。Ca2+动员和胞吐均表现出对拉伸刺激强度的剂量依赖性。因此，机械因素可以在非神经元、非肌肉细胞中触发复杂的细胞事件，并可能参与调节正常的肺功能。
Deep inflation of the lung stimulates surfatcant secretion by unknown mechanisms. The hypothesis that mechanical distension directly stimulates tuype II cells to secrete surfactant was tested by stretching type II cells cultured on silastic membranes. The intracellular Ca2+ concentration was measured in single cells, before and after stretching. A single stretch of alveolar type II cells caused a transient (less than 60 seconds) increase in cytosolic Ca2+ followed by a sustained (15 to 30 minutes) stimulation of surfactant secretion. Both Ca2+ mobilization and exocytosis exhibited dose-dependence to the magnitude of the stretch-stimulus. Thus, mechanical factors can trigger complex cellular events in nonneuron, nonmuscle cells and may be involved in regulating normal lung functions.