Matrix metalloproteinase activation predicts amelioration of remodeling after dietary modification in injured arteries.
Matrix metalloproteinase activation predicts amelioration of remodeling after dietary modification in injured arteries.
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DOI：
10.1161/atvbaha.110.216036
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Sadeghi MM
中科院分区：
文献类型：
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作者：
Tavakoli S;Razavian M;Zhang J;Nie L;Marfatia R;Dobrucki LW;Sinusas AJ;Robinson S;Edwards DS;Sadeghi MM
To establish and validate early non-invasive imaging of matrix metalloproteinase (MMP) activation for monitoring the progression of vascular remodeling and response to dietary modification. Apolipoprotein E−/− mice that were fed a high fat diet underwent left common carotid artery wire injury. One week after surgery, a group of animals were withdrawn from the high fat diet. The other group of animals continued that diet throughout the study. MicroSPECT/CT imaging with RP805 (a 99mTc-labeled tracer targeting activated MMPs) was repeatedly performed at 2 and 4 weeks after surgery. Histological analysis at 4 week showed significant left carotid neointima formation, monocyte/macrophage infiltration, and upregulation of several MMPs, which were ameliorated by withdrawal from the high fat diet. In vivo microSPECT/CT images visualized significant RP805 uptake, reflecting MMP activation, in the injured carotid arteries. MMP activation was reduced as early as 1 week after withdrawal from the high fat diet and significantly correlated with neointimal area at 4 weeks after surgery. MMP activation predicts the progression of vascular remodeling and can track the effect of dietary modification following vascular injury.
