In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy
In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy
复制标题
DOI：
10.1016/j.biomaterials.2008.12.001
复制
发表时间：
2009-03-01
期刊：
影响因子：
14
通讯作者：
Yang, Ke
中科院分区：
文献类型：
--
作者：
Xu, Liping;Pan, Feng;Yang, Ke
镁作为一种生物可吸收的生物材料已显示出潜在的应用，例如用于接骨螺钉和接骨板。以提高表面生物活性。通过磷化工艺在镁合金上涂覆磷酸钙（Ca-P涂层）。表面形貌分析表明，镁合金表面形成了多孔网状的Ca（4）中心点2 H（2）O层，并含有少量的Mg-21和Zn-21。在体外细胞实验中，L929细胞在Ca-P涂层镁合金上显示出显著良好的粘附性和显著高的生长速率和增殖特性（p < 0.05），表明Ca-P涂层显著改善了镁的表面细胞相容性。对钙磷涂层和裸合金棒进行了体内骨反应研究，以探讨早期骨反应。常规病理检查和免疫组化分析均表明，Ca-P涂层为镁提供了显著良好的表面生物活性（p < 0.05），并促进了种植体/骨界面的早期骨生长。结果表明，钙磷涂层是提高镁合金表面生物活性的有效方法。(C)2008爱思唯尔有限公司保留所有权利。
Magnesium has shown potential application as a bio-absorbable biomaterial, Such as for bone screws and plates. In order to improve the Surface bioactivity. a calcium phosphate was coated oil a magnesium alloy by a phosphating process (Ca-P coating). The Surface characterization showed that a porous and netlike CaGPO(4)center dot 2H(2)O layer with small amounts of Mg-21 and Zn-21 was formed oil the surface of the Mg alloy. Cells L929 showed significantly good adherence and significantly high growth rate and proliferation characteristics oil the Ca-P coated magnesium alloy (p < 0.05) in in-vitro cell experiments, demonstrating that the Surface cytocompatibility of magnesium was significantly improved by the Ca-P coating. In vivo implantations of the Ca-P coated and the naked alloy rods were carried Out to investigate the bone response at the early stage. Both routine pathological examination and immunohistochemical analysis demonstrated that the Ca-P coating provided magnesium with a significantly good Surface bioactivity (p < 0.05) and promoted early bone growth at the implant/bone interface. It Was suggested that the Ca-P coating might be all effective method to improve the surface bioactivity of magnesium alloy. (C) 2008 Elsevier Ltd. All rights reserved.