Clostridium perfringens urease genes are plasmid borne
Clostridium perfringens urease genes are plasmid borne
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DOI：
10.1128/iai.65.6.2313-2320.1997
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发表时间：
1997-06-01
影响因子：
3.1
通讯作者：
Cole, ST
中科院分区：
文献类型：
--
作者：
Dupuy, B;Daube, G;Cole, ST
虽然许多细菌是尿素分解的，并且在某些情况下脲酶作为毒力因子，但在厌氧病原体产气荚膜梭菌中尚未分析脲酶表型。在本研究中，与C.发现代表主要生物型的产气荚膜杆菌菌株具有尿素酶结构基因ureABC，并且这些基因定位于大质粒上，所述大质粒通常另外编码致死性β-或β-毒素或肠毒素。这代表了革兰氏阳性菌中质粒编码的尿素酶的首次报道。梭产气荚膜杆菌的酶与其他细菌的尿素酶高度相似，并与针对从幽门螺杆菌纯化的尿素酶产生的抗体发生交叉反应。尿素抑制脲酶的产生，并在氮源的可用性是有限的生长条件下诱导。到目前为止，这种形式的调节只观察到染色体ureABC基因。
Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. In this study, similar to 2% of C. perfringens strains, representing the principal biotypes, were found to harbor the urease structural genes, ureABC, and these were localized on large plasmids that often encode, in addition, the lethal epsilon or iota toxins or the enterotoxin. This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The C. perfringens enzyme was highly similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter pylori. Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was limiting. To date, this form of regulation has been observed only for chromosomal ureABC genes.