Misexpression of Polycomb-group proteins in Xenopus alters anterior neural development and represses neural target genes.
Misexpression of Polycomb-group proteins in Xenopus alters anterior neural development and represses neural target genes.
复制标题
非洲爪蟾中多梳族蛋白的错误表达会改变前神经发育并抑制神经靶基因。
DOI：
10.1006/dbio.1999.9473
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hollenberg,SM
中科院分区：
文献类型：
--
作者：
Yoshitake,Y;Howard,TL;Christian,JL;Hollenberg,SM
在果蝇中，Polycomb组构成了一组结构不同的蛋白质，它们共同作用以沉默靶基因。许多哺乳动物的多梳族蛋白也已被确定，并显示出与无脊椎动物的功能相似。为了开始分析多梳组蛋白在非洲爪蟾发育中的功能，我们克隆了果蝇多梳组蛋白的非洲爪蟾同源物XPcl 1。XPcl 1 mRNA在母体和合子中都存在，在前中枢神经系统中具有突出的合子表达。通过RNA注射到胚胎中的Pcl 1的错误表达在前中枢神经系统中产生缺陷。前脑和中脑包含以脑室为代价的多余神经组织，并且包括大大增厚的底板和顶板。眼区存在，但眼睛特异性标记物Rx 2A被完全抑制。在非洲爪蟾胚胎中Pcl 1的过度表达改变了两个后脑标记，抑制En-2并将其和Krox-20向后移动。类似的神经表型和En-2表达模式的影响，产生过表达的其他三个结构无关的Polycomb组蛋白：M33，XBmi-1，和mPh 2。这些观察结果表明Polycomb组在调节发育中的前中枢神经系统的基因表达方面发挥着重要作用。
In Drosophila, the Polycomb-group constitutes a set of structurally diverse proteins that act together to silence target genes. Many mammalian Polycomb-group proteins have also been identified and show functional similarities with their invertebrate counterparts. To begin to analyze the function of Polycomb-group proteins in Xenopus development, we have cloned a Xenopus homolog of Drosophila Polycomblike, XPcl1. XPcl1 mRNA is present both maternally and zygotically, with prominent zygotic expression in the anterior central nervous system. Misexpression of Pcl1 by RNA injection into embryos produces defects in the anterior central nervous system. The forebrain and midbrain contain excess neural tissue at the expense of the ventricle and include greatly thickened floor and roof plates. The eye fields are present but Rx2A, an eye-specific marker, is completely repressed. Overexpression of Pcl1 in Xenopus embryos alters two hindbrain markers, repressing En-2 and shifting it and Krox-20 in a posterior direction. Similar neural phenotypes and effects on the En-2 expression pattern were produced by overexpression of three other structurally unrelated Polycomb-group proteins: M33, XBmi-1, and mPh2. These observations indicate an important role for the Polycomb-group in regulating gene expression in the developing anterior central nervous system.