Constrained tibial vibration does not produce an anabolic bone response in adult mice
Constrained tibial vibration does not produce an anabolic bone response in adult mice
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DOI：
10.1016/j.bone.2009.06.025
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发表时间：
2009-10-01
期刊：
影响因子：
4.1
通讯作者：
Silva, Matthew J.
中科院分区：
文献类型：
--
作者：
Christiansen, Blaine A.;Kotiya, Akhilesh A.;Silva, Matthew J.
骨质疏松症的特点是骨量低和骨折风险增加。高频、低振幅全身振动（WBV）已被提议作为骨质疏松症的治疗方法，因为它可以刺激新骨形成并防止小梁骨丢失。我们开发了约束胫骨振动（CTV）作为小鼠小腿受控振动负载的方法。我们首先让小鼠接受五周的每日 CTV 负载（0.5 G 最大加速度），选择负载参数来独立研究应变大小、负载频率和循环加速度对振动自适应响应的影响。我们假设承受最大动态应变的小鼠将具有最大的骨形成反应。我们观察到 CTV 负载对骨小梁有轻微的局部益处，因为骨应变幅度最高的小鼠的负载胫骨与未负载胫骨的 BV/TV 高出 5.2%。然而，尽管存在这些积极的差异，我们观察到与假手术动物和基线对照动物相比，CTV 负载小鼠的负载和非负载胫骨中的小梁结构测量值显着较低，表明 CTV 油小梁骨的负面全身效应。基于这一证据，我们进行了一项后续研究，其中对小鼠进行 CTV 或假负荷，并在研究开始和结束时使用体内 microCT 扫描胫骨。与第一项研究的结果一致，CTV 加载小鼠和 Sham 小鼠的胫骨小梁 BV/TV 在第 36 天平均比第 0 天分别低 36% 和 31%，而同期年龄匹配对照小鼠则低 20%。与第一项研究相反，CTV 负载小鼠的负载和非负载胫骨之间没有差异，没有提供 CTV 局部益处的证据。总之，5周的每日CTV负荷对小鼠来说，对于胫骨近端的骨小梁来说，充其量是微弱的合成代谢，而每天的处理和暴露于麻醉与小梁骨和皮质骨的显着损失有关。我们得出的结论是，麻醉成年小鼠骨骼的直接振动负荷不是合成代谢的。 (c) 2009 Elsevier Inc. 保留所有权利。
Osteoporosis is characterized by low bone mass and increased fracture risk. High frequency, low-amplitude whole-body vibration (WBV) has been proposed as a treatment for osteoporosis because it can stimulate new bone formation and prevent trabecular bone loss. We developed constrained tibial vibration (CTV) as a method for controlled vibrational loading of the lower leg of a mouse. We first Subjected mice to five weeks of daily CTV loading (0.5 G maximum acceleration) with loading parameters chosen to independently investigate the effects of strain magnitude, loading frequency, and cyclic acceleration on the adaptive response to vibration. We hypothesized that mice subjected to the highest magnitude of dynamic strain would have the largest bone formation response. We observed a slight, local benefit of CTV loading on trabecular bone, as BV/TV was 5.2% higher in the loaded vs. non-loaded tibia of mice loaded with the highest bone strain magnitude. However, despite these positive differences, we observed significantly lower measures of trabecular structure in both loaded and non-loaded tibias from CTV loaded mice compared to Sham and Baseline Control animals, indicating a negative systemic effect of CTV oil trabecular bone. Based on this evidence, we conducted a follow-up Study wherein mice were Subjected to CTV or sham loading, and tibias were scanned at the beginning and end of the study period using in vivo microCT. Consistent with the findings of the first Study, trabecular BV/TV in both tibias of CTV loaded and Sham mice was, on average, 36% and 31% lower on day 36 than day 0, respectively, compared to 20% lower in Age-Matched Controls over the same time period. Contrary to the first Study, there were no differences between loaded and non-loaded tibias in CTV loaded mice, providing no evidence for a local benefit of CTV. In summary, 5 weeks of daily CTV loading of mice was, at best, weakly anabolic for trabecular bone in the proximal tibia, while daily handling and exposure to anesthesia was associated with significant loss of trabecular and cortical bone. We conclude that direct vibrational loading of bone in anesthetized, adult mice is not anabolic. (c) 2009 Elsevier Inc. All rights reserved.