Ligand-Free Fe3 O4 /CMCS Nanoclusters with Negative Charges for Efficient Structure-Selective Protein Adsorption.
Ligand-Free Fe3 O4 /CMCS Nanoclusters with Negative Charges for Efficient Structure-Selective Protein Adsorption.
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DOI：
10.1002/smll.201600022
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发表时间：
2016-05
期刊：
影响因子：
13.3
通讯作者：
Qi Yang;Yue Zhu;Minggang Yang;Shaohua Ma;Yao Wu;Fang Lan;Z. Gu
中科院分区：
文献类型：
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作者：
Qi Yang;Yue Zhu;Minggang Yang;Shaohua Ma;Yao Wu;Fang Lan;Z. Gu
简单有效地从复杂混合物中捕获单个蛋白质在蛋白质组学和诊断学中具有重要意义。然而，吸附纳米材料通常要通过一个复杂而艰巨的过程来修饰特定的配体。Fe3O4/羧甲基壳聚糖(Fe3O4/CMCS)纳米团簇是一种新的非配基修饰策略，可以选择性地捕获牛血球蛋白(BHB)和其他结构相似的蛋白质(如溶菌酶(Lyz)和糜蛋白酶(CTP))。无配体Fe3O4/CMCS纳米团簇除了具有简单、经济的两步制备工艺外，还具有许多优点，包括：形貌均匀、高负电荷(-27 mV)、高饱和磁化强度(60emu g(-1))和高磁含量(85%)。此外，没有配体的Fe3O4/CMCS纳米簇被发现即使在生物样品中也能选择性地捕获模型蛋白质混合物中的BHB。从纳米材料和蛋白质结构两个方面进一步探讨了选择性捕获蛋白质的原因。在纳米材料方面，发现较高的负电荷有利于BHB的选择性吸附。考虑到蛋白质的结构，有趣的是，无配体磁性纳米团簇具有结构选择性的蛋白质吸附能力，可以有效地捕获结构类似于BHB的其他蛋白质，如LyZ和CTP，显示出无配体策略在生物医学领域的巨大潜力。
The easy and effective capture of a single protein from a complex mixture is of great significance in proteomics and diagnostics. However, adsorbing nanomaterials are commonly decorated with specific ligands through a complicated and arduous process. Fe3 O4 /carboxymethylated chitosan (Fe3 O4 /CMCS) nanoclusters are developed as a new nonligand modified strategy to selectively capture bovine hemoglogin (BHB) and other structurally similar proteins (i.e., lysozyme (LYZ) and chymotrypsin (CTP)). The ligand-free Fe3 O4 /CMCS nanoclusters, in addition to their simple and economical two-step preparation process, possess many merits, including uniform morphology, high negative charges (-27 mV), high saturation magnetization (60 emu g(-1) ), and high magnetic content (85%). Additionally, the ligand-free Fe3 O4 /CMCS nanoclusters are found to selectively capture BHB in a model protein mixture even within biological samples. The reason for selective protein capture is further investigated from nanomaterials and protein structure. In terms of nanomaterials, it is found that high negative charges are conducive to selectively adsorb BHB. In consideration of protein structure, interestingly, the ligand-free magnetic nanoclusters display a structure-selective protein adsorption capacity to efficiently capture other proteins structurally similar to BHB, such as LYZ and CTP, showing great potential of the ligand-free strategy in biomedical field.