In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles
In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles
复制标题
使用倒置膜囊泡进行体外鞭毛 III 型蛋白质转运测定
DOI：
10.1007/978-1-0716-3060-0_2
复制
发表时间：
2023
影响因子：
--
通讯作者：
Terashima Hiroyuki
中科院分区：
文献类型：
--
作者：
Imada Katsumi;Terashima Hiroyuki
鞭毛轴蛋白通过鞭毛蛋白输出器穿过细胞膜进入生长鞭毛的中央通道，鞭毛蛋白输出器是III型分泌系统(T3SS)的成员。为了揭示T3SS转运蛋白质的分子机制，准确测量各种条件下的蛋白质转运是必不可少的。在这一章中，我们描述了一种利用沙门氏菌制备的倒置膜泡(IMV)进行鞭毛蛋白转运分析的体外方法。该方法可以方便、准确地控制T3SS周围的情况，并可应用于其他T3SS。
The flagellar axial proteins are transported across the cytoplasmic membrane into the central channel of the growing flagellum via the flagellar protein export apparatus, a member of the type III secretion system (T3SS). To reveal the molecular mechanism of protein transport by the T3SS, accurate measurement of protein transport under various conditions is essential. In this chapter, we describe an in vitro method for flagellar protein transport assay using inverted membrane vesicles (IMVs) prepared fromSalmonellacells. This method can easily and precisely control the condition around the T3SS and be applied to other T3SSs.